Paris/August 7
From 2007.igem.org
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|width=50%| Description | |width=50%| Description | ||
|width=15%| Products in use | |width=15%| Products in use | ||
| - | |||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP1 | | style="background: #ccffcc;" |MP1 | ||
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|Strong constitutive promoter in J61002 (clones 1 + 2) | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| | | | ||
| - | | | + | |- style="background: #cccccc;" |
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP1 | ||
| + | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
| + | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| + | | | ||
| + | |} | ||
Revision as of 18:21, 7 August 2007
Study of the pBAD promoter
We've put the AraC-pBAD promoter (BBa_I0500) in the expression vector BBa_J61002 (between EcoRI and SpeI)
Digestion reactions
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D37'.1 | lox71-gfp(tripart) BI | L12.1 miniprep product (from 30/07 ligation reaction) | XbaI | PstI | lox71_RBS-rfp-double terminator extracted as a backward insert | ||||
| D39' | BI-insert lox66-DapAE.coli | lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2) | XbaI | PstI | lox66-dapAcoli extracted for usage as a backward insert | ||||
| D41 | araC/pBad-J61002 plasmid SP dig. | L1.2, L1".1 & L1".2 miniprep product | SpeI | PstI | J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS | ||||
Minipreps
| Number | Name | Description | Products in use |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) |
