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Revision as of 18:21, 7 August 2007 (view source) Landrain (Talk | contribs) (→Minipreps) ← Older edit		Revision as of 18:31, 7 August 2007 (view source) Landrain (Talk | contribs) (→Minipreps) Newer edit →
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	|width=15%| Products in use		|width=15%| Products in use
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT2
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|S16.2
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT3.1
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P6.1
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT3.2
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P6.7
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT4.1
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P8.2
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT4.2
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P8.5
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT4.3
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P8.7
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT4.4
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P8.8
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT4.5
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P8.10
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT5.1
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P9.1
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT5.2
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P9.2
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
-	| style="background: #ccffcc;" |MP1	+	| style="background: #ccffcc;" |MPT5.3
-	|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>	+	|
-	|Strong constitutive promoter in J61002 (clones 1 + 2)	+	|pTOPO P9.4
	|		|
	|}		|}

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Revision as of 18:31, 7 August 2007

Study of the pBAD promoter

We've put the AraC-pBAD promoter (BBa_I0500) in the expression vector BBa_J61002 (between EcoRI and SpeI)

Digestion reactions

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Size	Description
D37'.1	lox71-gfp(tripart) BI	L12.1 miniprep product (from 30/07 ligation reaction)	XbaI	PstI		lox71_RBS-rfp-double terminator extracted as a backward insert
D39'	BI-insert lox66-DapAE.coli	lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)	XbaI	PstI		lox66-dapAcoli extracted for usage as a backward insert
D41	araC/pBad-J61002 plasmid SP dig.	L1.2, L1".1 & L1".2 miniprep product	SpeI	PstI		J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS

Minipreps

Number	Name	Description	Products in use
MPT2		S16.2
MPT3.1		pTOPO P6.1
MPT3.2		pTOPO P6.7
MPT4.1		pTOPO P8.2
MPT4.2		pTOPO P8.5
MPT4.3		pTOPO P8.7
MPT4.4		pTOPO P8.8
MPT4.5		pTOPO P8.10
MPT5.1		pTOPO P9.1
MPT5.2		pTOPO P9.2
MPT5.3		pTOPO P9.4