Edinburgh/DivisionPopper/Applications
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===Counting using more recombination sections=== | ===Counting using more recombination sections=== | ||
| - | Rather than using the DivisionPoPper directly, this uses | + | Rather than using the DivisionPoPper directly, this uses flipping dif sites to activate different recombinases, cut out sections of DNA and thus enable a range of downstream functions with each division. Functions are represented by fluorescent reporter genes here for sake of visibility, but may be replaced with genes to execute a function of choice; e g metabolite receptors and directed cellular movement. |
[[Image:Cell division.jpg|800px]] | [[Image:Cell division.jpg|800px]] | ||
[[Edinburgh/DivisionPopper| Introduction]] | [[Edinburgh/DivisionPopper/Applications|Applications]] | [[Edinburgh/DivisionPopper/Design|Design]] | [[Edinburgh/DivisionPopper/Status|Status]] | [[Edinburgh/DivisionPopper/References|References]] | [[Edinburgh/DivisionPopper| Introduction]] | [[Edinburgh/DivisionPopper/Applications|Applications]] | [[Edinburgh/DivisionPopper/Design|Design]] | [[Edinburgh/DivisionPopper/Status|Status]] | [[Edinburgh/DivisionPopper/References|References]] | ||
Revision as of 19:49, 8 August 2007
Edinburgh > DivisionPopper
Introduction | Applications | Design | Status | References
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Applications and further research
Contents |
This page details some of the potential uses of the Division PoPper and other ways of using the recombination systems
Division Frequency Analysis
The output of the Division PoPper could be linked to the production of a slowly degrading protein. The more frequent the divisions, the greater the concentration of the protein.
Division Counting
Coupling to a PoPS counting device
Couple the output of the Division PoPper to another counting device (such as the ETH Zurich counter or other variants) to count the number of cell divisions. This is difficult to test due to the nature of colonies and cells dividing out of phase. We get around this problem by using high-power microscopy to study the activity of single cells.
Counting using more recombination sections
Rather than using the DivisionPoPper directly, this uses flipping dif sites to activate different recombinases, cut out sections of DNA and thus enable a range of downstream functions with each division. Functions are represented by fluorescent reporter genes here for sake of visibility, but may be replaced with genes to execute a function of choice; e g metabolite receptors and directed cellular movement.
Introduction | Applications | Design | Status | References
