From 2007.igem.org

Revision as of 20:31, 27 June 2007 (view source) Vaibhaviu (Talk | contribs) ← Older edit		Latest revision as of 20:25, 8 August 2007 (view source) Vaibhaviu (Talk | contribs)
(One intermediate revision not shown)
Line 1:		Line 1:
	<pre>		<pre>
-	Biobrickification of composite part: RBS (pBca 9145 – Bca1090) + katG, HPI gene	+
-	---------------------------------------------------	+	Method Used: dBBS
-	Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L)	+
-	Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L)	+	PCR ca1099F/G00101 on Part A (pBca9145-Bca1090) (553 bp)
		+	Digest pBca9145-Bca1090 (BsaI/BamHI, 989+1102, S)
	Product is I716258		Product is I716258
-	---------------------------------------------------	+	Ligate with Part B
-	No Oligos Needed	+	-------------------------------------
		+	ca1099F – ccaaaAGATCTatgaaacgttttagtctggc
		+	G00101 – gctagCTCGAGttaGGATCCttacttaattacaccacaggc
		+
		+
		+	PCR ca998/ca1099R on Part B (pBca9145-I716253) (3393 bp)
		+	Digest pBca9145-I716253 (BsaI/BglII, 2512 + 777, L)
		+	Product is I716259
		+	Ligate with Part A
		+	-------------------------------------
		+	ca998 – gtatcacgaggcagaatttcag
		+	ca1099R – gctgataaatctggagccgg
		+
		+	Final product is I716260
		+
	</pre>		</pre>

---

Latest revision as of 20:25, 8 August 2007

Method Used: dBBS

PCR ca1099F/G00101 on Part A (pBca9145-Bca1090)	(553 bp)
Digest pBca9145-Bca1090				(BsaI/BamHI, 989+1102, S)
Product is I716258
Ligate with Part B
-------------------------------------
ca1099F – ccaaaAGATCTatgaaacgttttagtctggc
G00101 – gctagCTCGAGttaGGATCCttacttaattacaccacaggc


PCR ca998/ca1099R on Part B (pBca9145-I716253)	(3393 bp)
Digest pBca9145-I716253				(BsaI/BglII, 2512 + 777, L)
Product is I716259
Ligate with Part A
-------------------------------------
ca998 – gtatcacgaggcagaatttcag
ca1099R – gctgataaatctggagccgg

Final product is I716260