BerkiGEM2007-AustinConstructionFile55
From 2007.igem.org
(Difference between revisions)
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Quickchange AD0015F/R to get rid of XhoI site. <br> | Quickchange AD0015F/R to get rid of XhoI site. <br> | ||
<br> | <br> | ||
- | PCR AD0016F/R on modified I716092. | + | PCR AD0016F/R on modified I716092.<br> |
- | Digest Bgl2/Xho1 and insert into 9145 plasmid. | + | Digest Bgl2/Xho1 and insert into 9145 plasmid.<br> |
- | Product is I716355 | + | Product is I716355<br> |
Latest revision as of 01:50, 9 August 2007
Pre-biobricking modifications to I716092.
Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.
PCR AD0016F/R on modified I716092.
Digest Bgl2/Xho1 and insert into 9145 plasmid.
Product is I716355