NYMU Taipei/Lab Notes/2007 8 8

< NYMU Taipei/Lab Notes(Difference between revisions)

Latest revision as of 13:07, 9 August 2007

@@ Line 1: / Line 1: @@
 * Attendant
-** 海威, 香君, 德銘, 威超, 志賢
+** 海威, 香君, 宥嫺, 德銘, 威超, 志賢
-* Transformation
+* References
+** [https://2006.igem.org/wiki/index.php/Assembly BioBrick Assembly]
+* Transformation to competent cell (PM 7:30)(Add <font color="red">1uL</font> vector to 50uL ECOS competent cells)
 ** OmpR binding promoter
-*** [http://partsregistry.org/Part:BBa_R0082 Part::BBa_R0082, three OmpR binding operator sites C1, C2, C3 (from OmpC)] at 15P, plate 1
+*** [http://partsregistry.org/Part:BBa_R0082 Part::BBa_R0082, three OmpR binding operator sites C1, C2, C3 (from OmpC)] at 15P, plate 1 (by 海威)
 *** [http://partsregistry.org/Part:BBa_R0083 Part::BBa_R0083, only one OmpR binding operator site C1 (truncated version of BBa_R0082)]
 *** [http://partsregistry.org/Part:BBa_R0084 Part::BBa_R0084, three OmpR binding operator sites F1, F2, F3 (from OmpF)]
+** High Transcription promoter
+*** [http://partsregistry.org/Part:BBa_I14032 Part:BBa_I14032, Constitutive Promoter, High Transcription] at 17K, plate 2 (by 威超)
 * cDNA clones
-** INS [[Image:IMAGE 3950204.png]]
+** INS
-** IGFBP3 [[Image:IMAGE 3632247.png]]
+*** [[Image:IMAGE 3950204.png]]
-** IGFPB7
+** IGFBP7
+*** [[Image:IMAGE 3632247.png]]
+** IGFPB3
+*** [[Image:IMAGE 3506929.png]]