NYMU Taipei/Lab Notes/2007 8 8
From 2007.igem.org
< NYMU Taipei/Lab Notes(Difference between revisions)
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* Attendant | * Attendant | ||
| - | ** 海威, 香君, 德銘, 威超, 志賢 | + | ** 海威, 香君, 宥嫺, 德銘, 威超, 志賢 |
| - | * Transformation | + | * References |
| + | ** [https://2006.igem.org/wiki/index.php/Assembly BioBrick Assembly] | ||
| + | * Transformation to competent cell (PM 7:30)(Add <font color="red">1uL</font> vector to 50uL ECOS competent cells) | ||
** OmpR binding promoter | ** OmpR binding promoter | ||
| - | *** [http://partsregistry.org/Part:BBa_R0082 Part::BBa_R0082, three OmpR binding operator sites C1, C2, C3 (from OmpC)] at 15P, plate 1 | + | *** [http://partsregistry.org/Part:BBa_R0082 Part::BBa_R0082, three OmpR binding operator sites C1, C2, C3 (from OmpC)] at 15P, plate 1 (by 海威) |
*** [http://partsregistry.org/Part:BBa_R0083 Part::BBa_R0083, only one OmpR binding operator site C1 (truncated version of BBa_R0082)] | *** [http://partsregistry.org/Part:BBa_R0083 Part::BBa_R0083, only one OmpR binding operator site C1 (truncated version of BBa_R0082)] | ||
*** [http://partsregistry.org/Part:BBa_R0084 Part::BBa_R0084, three OmpR binding operator sites F1, F2, F3 (from OmpF)] | *** [http://partsregistry.org/Part:BBa_R0084 Part::BBa_R0084, three OmpR binding operator sites F1, F2, F3 (from OmpF)] | ||
| + | ** High Transcription promoter | ||
| + | *** [http://partsregistry.org/Part:BBa_I14032 Part:BBa_I14032, Constitutive Promoter, High Transcription] at 17K, plate 2 (by 威超) | ||
* cDNA clones | * cDNA clones | ||
** INS | ** INS | ||
Latest revision as of 13:07, 9 August 2007
- Attendant
- 海威, 香君, 宥嫺, 德銘, 威超, 志賢
- References
- Transformation to competent cell (PM 7:30)(Add 1uL vector to 50uL ECOS competent cells)
- OmpR binding promoter
- [http://partsregistry.org/Part:BBa_R0082 Part::BBa_R0082, three OmpR binding operator sites C1, C2, C3 (from OmpC)] at 15P, plate 1 (by 海威)
- [http://partsregistry.org/Part:BBa_R0083 Part::BBa_R0083, only one OmpR binding operator site C1 (truncated version of BBa_R0082)]
- [http://partsregistry.org/Part:BBa_R0084 Part::BBa_R0084, three OmpR binding operator sites F1, F2, F3 (from OmpF)]
- High Transcription promoter
- [http://partsregistry.org/Part:BBa_I14032 Part:BBa_I14032, Constitutive Promoter, High Transcription] at 17K, plate 2 (by 威超)
- OmpR binding promoter
- cDNA clones
- INS
-
- IGFBP7
-
- IGFPB3
-
- INS
