NYMU Taipei/Lab Notes/2007 8 8

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* Attendant
* Attendant
-
** 海威, 香君, 幼嫻, 德銘, 威超, 志賢  
+
** 海威, 香君, 宥嫺, 德銘, 威超, 志賢
-
* Transformation to competent cell
+
* References
 +
** [https://2006.igem.org/wiki/index.php/Assembly BioBrick Assembly]
 +
* Transformation to competent cell (PM 7:30)(Add <font color="red">1uL</font> vector to 50uL ECOS competent cells)
** OmpR binding promoter
** OmpR binding promoter
-
*** [http://partsregistry.org/Part:BBa_R0082 Part::BBa_R0082, three OmpR binding operator sites C1, C2, C3 (from OmpC)] at 15P, plate 1
+
*** [http://partsregistry.org/Part:BBa_R0082 Part::BBa_R0082, three OmpR binding operator sites C1, C2, C3 (from OmpC)] at 15P, plate 1 (by 海威)
*** [http://partsregistry.org/Part:BBa_R0083 Part::BBa_R0083, only one OmpR binding operator site C1 (truncated version of BBa_R0082)]
*** [http://partsregistry.org/Part:BBa_R0083 Part::BBa_R0083, only one OmpR binding operator site C1 (truncated version of BBa_R0082)]
*** [http://partsregistry.org/Part:BBa_R0084 Part::BBa_R0084, three OmpR binding operator sites F1, F2, F3 (from OmpF)]
*** [http://partsregistry.org/Part:BBa_R0084 Part::BBa_R0084, three OmpR binding operator sites F1, F2, F3 (from OmpF)]
** High Transcription promoter
** High Transcription promoter
-
*** [http://partsregistry.org/Part:BBa_I14032 Part:BBa_I14032, Constitutive Promoter, High Transcription] at 17K, plate 2
+
*** [http://partsregistry.org/Part:BBa_I14032 Part:BBa_I14032, Constitutive Promoter, High Transcription] at 17K, plate 2 (by 威超)
* cDNA clones
* cDNA clones
** INS
** INS

Latest revision as of 13:07, 9 August 2007

  • Attendant
    • 海威, 香君, 宥嫺, 德銘, 威超, 志賢
  • References
  • Transformation to competent cell (PM 7:30)(Add 1uL vector to 50uL ECOS competent cells)
    • OmpR binding promoter
      • [http://partsregistry.org/Part:BBa_R0082 Part::BBa_R0082, three OmpR binding operator sites C1, C2, C3 (from OmpC)] at 15P, plate 1 (by 海威)
      • [http://partsregistry.org/Part:BBa_R0083 Part::BBa_R0083, only one OmpR binding operator site C1 (truncated version of BBa_R0082)]
      • [http://partsregistry.org/Part:BBa_R0084 Part::BBa_R0084, three OmpR binding operator sites F1, F2, F3 (from OmpF)]
    • High Transcription promoter
      • [http://partsregistry.org/Part:BBa_I14032 Part:BBa_I14032, Constitutive Promoter, High Transcription] at 17K, plate 2 (by 威超)
  • cDNA clones
    • INS
      • IMAGE 3950204.png
    • IGFBP7
      • IMAGE 3632247.png
    • IGFPB3
      • IMAGE 3506929.png
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