BerkiGEM2007-AustinConstructionFile53
From 2007.igem.org
(Difference between revisions)
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PCR AD0012F/AD0012R on K-12 strain genome (2701 bp)<br> | PCR AD0012F/AD0012R on K-12 strain genome (2701 bp)<br> | ||
- | Post PCR digest with (NdeI/ | + | Post PCR digest with (NdeI/XhoI/DpnI) <br> |
- | Digest petDUET (NdeI/ | + | Digest petDUET (NdeI/XhoI, 5412/8, Larger)<br> |
Ligation product is I716353<br> | Ligation product is I716353<br> |
Latest revision as of 01:28, 11 August 2007
PCR AD0012F/AD0012R on K-12 strain genome (2701 bp)
Post PCR digest with (NdeI/XhoI/DpnI)
Digest petDUET (NdeI/XhoI, 5412/8, Larger)
Ligation product is I716353