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Revision as of 15:19, 30 July 2007 (view source) Davidpz (Talk | contribs) (→ON Bacterial cultures) ← Older edit		Revision as of 09:31, 13 August 2007 (view source) Nicolas C. (Talk | contribs) (→FtsZ<sup>TS</sup> strain screening) Newer edit →
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	** One at 30°C		** One at 30°C
	** One at 42°C		** One at 42°C
-	Results : every strain grew at 30 and 42°C => fail.	+	Results : every strain grew at 30 and 42°C => FAIL
-	>br	+
-	>br	+
	== Overnight Bacterial cultures ==		== Overnight Bacterial cultures ==
	We launched several cultures :		We launched several cultures :

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Revision as of 09:31, 13 August 2007

FtsZ^TS strain screening

We want to test these strains :

• 121.4
• 121.5
• 121.6
• 129
• 1.129
• DCR 14.1
• DCR 14.2
• DCR 14.3

I made glycerol stock of these strains, stored in the freezer. Test :

• Spread these strains on two plates (preheated before spreading) :
  • One at 30°C
  • One at 42°C

Results : every strain grew at 30 and 42°C => FAIL

Overnight Bacterial cultures

We launched several cultures :

• MG1655 -> to do transductions tomorrow (don't forget the DAP, David!)
• pJ23107_J61002 clones 3 & 4 in LB-Amp liquid; also striping clones 3-18 on LB-Amp Agar plates
• I0500_pSB2K3 clones 1 & 2 in LB-Kan liquid medium
• B0015_pSB1AK3 clones 3 & 4 in LB-Kan liquid medium

Tomorrow (20/07/07): perform transduction (MG1655), minipreps, glycerol stocks