Paris/August 16

(Difference between revisions)

Revision as of 01:17, 17 August 2007

The cloning finally seem to have worked, so we are doing colony PCRs to check if there is an insert and if it's got the right size.

The following digestion reactions (ON) were performed:

PURPLE = Vector
GREEN = Insert

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Size	Description			Date
D37	lox71-gfp(tripart) BI	L12.2 miniprep products (from 30/07 ligation reaction)	XbaI	PstI		lox71_RBS-gfp-double terminator extracted as a backward insert			Aug. 16

I was unable to perform the following digestion reactions (because their was no more MP1 or MP2 product left):

PURPLE = Vector
GREEN = Insert

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Size	Description	Date
D1	pJ23100	pJ23100	Spe1	Pst1	2096	Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
D3	pJ23107	pJ23107	Spe1	Pst1	2096	Vector with the J23107 promoter ready for insertion of a RBS/CDS insert

@@ Line 9: / Line 9: @@
 The following digestion reactions (ON) were performed:
+<br>
+<font color="#FF99FF" size="4">PURPLE = Vector </font><br>
+<font color="#ccffcc" size="4">GREEN = Insert </font><br>
+{|
+|- style="background: #ccccff;"
+! colspan="5" style="background: #ccccff;" | Digestion Products
+|- style="background: #ccccff; text-align: center;"
+|width=5%| Number
+|width=24%| Product Name
+|width=20%| Matrix Name
+|width=5%| Enzyme 1
+|width=5%| Enzyme 2
+|width=5%| Size
+|width=34%| Description
+|width=1%|
+|width=1%|
+|width=5%| Date
+|- style="background: #cccccc;"
+| style="background: #ccffcc;" |D37
+|lox71-gfp(tripart) BI
+|L12.2 miniprep products (from [[Paris/July 30| 30/07]] ligation reaction)
+|XbaI
+|PstI
+|
+|lox71_RBS-gfp-double terminator extracted as a backward insert
+|
+|
+|Aug. 16
+|}
+I was unable to perform the following digestion reactions (because their was no more MP1 or MP2 product left):
 <br>
@@ Line 37: / Line 72: @@
 |
 |
-|Aug. 16
+|
 |- style="background: #cccccc;"
 | style="background: #FF99FF;" |D3
@@ Line 48: / Line 83: @@
 |
 |
-|Aug. 16
-|- style="background: #cccccc;"
-| style="background: #ccffcc;" |D37
-|lox71-gfp(tripart) BI
-|L12.2 miniprep products (from [[Paris/July 30| 30/07]] ligation reaction)
-|XbaI
-|PstI
 |
-|lox71_RBS-gfp-double terminator extracted as a backward insert
-|
-|
-|Aug. 16
 |}