NYMU Taipei/Primer List
From 2007.igem.org
(Difference between revisions)
Lihsiangyen (Talk | contribs) |
Lihsiangyen (Talk | contribs) |
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|M_INS_A 3' | |M_INS_A 3' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font> |
| - | | | + | |48 bp |
|54.74 | |54.74 | ||
|SpeI, PstI | |SpeI, PstI | ||
|126 bp | |126 bp | ||
| - | |Potential dimer formation! | + | |Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
|- | |- | ||
|M_INS_B 5' | |M_INS_B 5' | ||
| Line 35: | Line 35: | ||
|- | |- | ||
|M_INS_B 3' | |M_INS_B 3' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GGACATGGGTGTGTAGAAGA</font> |
| - | | | + | |48 bp |
|55.86 | |55.86 | ||
|SpeI, PstI | |SpeI, PstI | ||
|156 bp | |156 bp | ||
| - | |Potential dimer formation! | + | |Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
|- | |- | ||
|- | |- | ||
| Line 53: | Line 53: | ||
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|H_INS_A 3' | |H_INS_A 3' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GTTGCAGTAGTTCTCCAGCTG</font> |
| - | | | + | |49 bp |
|57.77 | |57.77 | ||
|SpeI, PstI | |SpeI, PstI | ||
|129 bp | |129 bp | ||
| - | |Potential dimer formation! | + | |Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
|- | |- | ||
|H_INS_B 5' | |H_INS_B 5' | ||
| Line 70: | Line 70: | ||
|- | |- | ||
|H_INS_B 3' | |H_INS_B 3' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GGTCTTGGGTGTGTAGAAGA</font> |
| - | | | + | |48 bp |
|55.10 | |55.10 | ||
|SpeI, PstI | |SpeI, PstI | ||
|156 bp | |156 bp | ||
| - | | | + | |Stop codons are removed to enable fusion with export signal. |
|- | |- | ||
|- | |- | ||
Revision as of 07:05, 23 August 2007
| Name | Sequence | Length | Tm (C) | Restriction site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG | 51 bp | 56.85 | EcoRI, XbaI | 126 bp | Potential dimer formation! |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT | 48 bp | 54.74 | SpeI, PstI | 126 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| M_INS_B 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT | 52 bp | 60.85 | EcoRI, XbaI | 156 bp | Potential dimer formation! |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA | 48 bp | 55.86 | SpeI, PstI | 156 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTGGAACAATGCTG | 51 bp | 62.45 | EcoRI, XbaI | 129 bp | Potential dimer formation! |
| H_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG | 49 bp | 57.77 | SpeI, PstI | 129 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_B 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTGAACCAACACCTGTG | 51 bp | 57.98 | EcoRI, XbaI | 156 bp | Potential dimer formation! |
| H_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA | 48 bp | 55.10 | SpeI, PstI | 156 bp | Stop codons are removed to enable fusion with export signal. |
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1404 bp | |
| RcsC 3'-2 | GGGGTATACCAACGACTCTTCCATCTTCAC | 29 bp | 59.63 | NdeI | 1404 bp | Three extra guanosine was added to the 5' side of the NdeI cutting site. |
| [http://partsregistry.org/Part:BBa_G00100 VF2] | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| [http://partsregistry.org/Part:BBa_G00101 VR] | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. |
- [http://www.mdbio.com.tw/pfu.htm pfu primer]
