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	==Bacterial Targeting==		==Bacterial Targeting==
-		+	The motivation for this part of the project was two-fold: first, to make bacteria adhere to targets with a high degree of specificity, and second, to transduce a signal upon adhesion to their targets. Initial targeting screens were done with histidine and strep2 tags to their known nickel and streptavidin targets; after characterization and high enrichment with these known substrates, random libraries were inserted into the outer membrane proteins OmpA and AIDA. As we proceed with this experiment, we hope to characterize sequences that lend specificity to calmodulin and EGF.
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	==Quorum Sensing==		==Quorum Sensing==

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Revision as of 15:41, 23 August 2007

Harvard University 2007 iGEM Team
	Students Ellenor Brown Stephanie Lo Alexander Pickett Sammy Sambu Kevin Shee Perry Tsai Shaunak Vankudre George Xu Education Advisor Tamara Brenner	Teaching Fellows Nicholas Guido Bill Senapedis Mike Strong Harris Wang Faculty Advisors Debra Auguste George Church Jagesh Shah William Shih Pamela Silver Alain Viel

The Harvard University 2007 iGEM Team

This year Harvard's team consisted of 8 undergraduate students, with backgrounds in molecular and cellular biology, biochemistry, and computer science. With the help of 6 faculty advisers and 4 teaching fellows, plus one education advisor, they devised and executed a single project with three subsections.

Cling-E. Coli

1. Bacterial Targeting
2. Quorum Sensing
3. Two-Component Systems

Bacterial Targeting

The motivation for this part of the project was two-fold: first, to make bacteria adhere to targets with a high degree of specificity, and second, to transduce a signal upon adhesion to their targets. Initial targeting screens were done with histidine and strep2 tags to their known nickel and streptavidin targets; after characterization and high enrichment with these known substrates, random libraries were inserted into the outer membrane proteins OmpA and AIDA. As we proceed with this experiment, we hope to characterize sequences that lend specificity to calmodulin and EGF.

Quorum Sensing

Two-Component Systems

Click the picture, or click [http://openwetware.org/wiki/IGEM:Harvard here] for the more detailed Harvard iGEM 2007 wiki.