Davidson Missouri W

From 2007.igem.org

Revision as of 16:03, 17 May 2007

===Davidson & Missouri Western Team Logo, iGEM2006===

Western Meeting Notes

5/14 Overview of goals for the next two weeks

Begin Experimentation

Educational Information

Literature Research

Anything accomplished during this time is gravy.

5/15

Reviewed PowerPoint presentations of 2006 project to gain an understanding of the Pancake Problem.

Viewed Bruce's presentation on the mutation of the hix sites

Developed plan to build testing constructs of mutant hix sites, arriving this afternoon from Davidson by FedEx

Will build pLac - hix(n) - BBa_S03644 (RFPrev-RBSrev) - hix(n) on AmpR plasmid. Details to follow.

Note -- The above plan has changed as of Tuesday afternoon because of availability of parts.

BBa_S03644 is not available in our lab and the exact forward equivalent appears (amazingly) to not exist anywhere. However, there is an RBS - RFP in the Registry (BBa_I13502) which is a preassembled combination of a weaker RBS (BBa_B0034) and RFP (BBa_E1010). There is also a full positive control assembly (BBa_J04450) of pLac - B0034 - E1010 - Term. We will need to be careful to use the same parts in our Hix(n) assemblies so that we can test for only the functionality of our construct with the mutated Hix sites included.

5/16

We have cultures that were grown overnight of the Hix(n). We received the sequencing data on the Karen's and Bruce's Hix(n) constructs (from 5/14 report) and are analyzing the data to find the mutations. Lab work continues.

Students

• Lane H. Heard, Central High School graduate, [1]

• Michelle Ritter, junior mathematics major, [2]

• Kelly J. Malloy, senior chemistry major (health professions), [3]

• Brad J. Ogden, senior biology major (health sciences), [4]

Faculty

• Todd Eckdahl [http://staff.missouriwestern.edu/~eckdahl/], Professor, Department of Biology, [5]

• Jeff Poet [http://staff.missouriwestern.edu/~poet/], Assistant Professor, Department of Computer Science, Mathematics, and Physics, [6]

Shipping Address: Todd Eckdahl, Biology Department, Missouri Western State University, 4525 Downs Drive, Saint Joseph, MO, 64507 [(816) 271-5873]

• Malcolm Campbell [http://www.bio.davidson.edu/people/macampbell/macampbell.html], Professor, Department of Biology, [7]

• Karmella Haynes [http://www.bio.davidson.edu/people/kahaynes/kahaynes.html], Visiting Assistant Professor, Department of Biology, [8]

• Laurie Heyer [http://www.davidson.edu/math/heyer/], Associate Professor, Department of Mathematics, [9]

Shipping Address: Malcolm Campbell, Biology Dept. Davidson College, 209 Ridge Road, Davidson, NC 28036 [(704) 894-2692]

Project Overview

Solving the Pancake Problem with an E. coli Computer Our goal is to genetically engineer a biological system that can compute solutions to a puzzle called the burnt pancake problem. The EHOP computer is a proof of concept for computing in vivo, with implications for future data storage devices and transgenic systems. Our work was done in collaboration with the Davidson College iGEM team.

Resources / Citations

Cool site for Breakfast [http://www.cut-the-knot.org/SimpleGames/Flipper.shtml]

Karen Acker's paper describing GFP and TetA(c) with Hix insertions [http://www.bio.davidson.edu/Courses/Immunology/Students/spring2006/Acker/Acker_finalpaperGFP.doc]

Bruce Henschen's paper describing one-time flippable Hix sites [http://www.bio.davidson.edu/Courses/genomics/2006/henschen/Bruce_Finalpaper.doc]

Intro to Hamiltonian Path Problem and DNA [http://www.ams.org/featurecolumn/archive/dna-abc2.html]