NYMU Taipei/Primer List
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|RcsC 3'-2 | |RcsC 3'-2 | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCCATATG</font><font color="red">CAACGACTCTTCCATCTTCAC</font> |
|34 bp | |34 bp | ||
|59.63 | |59.63 | ||
Revision as of 16:37, 24 August 2007
Project Description /
Lab Notes /
Part List /
Primer List
| Name | Sequence | Length | Tm (C) | RE site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGGGCATTGTAGATCAGTGCTG | 54 bp | 56.85 | XbaI | 119 bp | Potential dimer formation! XbaI+RBS |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT | 48 bp | 54.74 | SpeI, PstI | 119 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| M_INS_B 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGTTTGTCAAGCAGCACCTTTGT | 55 bp | 60.85 | XbaI | 149 bp | Potential dimer formation! |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA | 48 bp | 55.86 | SpeI, PstI | 149 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_A 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGGGCATTGTGGAACAATGCTG | 54 bp | 62.45 | XbaI | 122 bp | Potential dimer formation! XbaI+RBS |
| H_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG | 49 bp | 57.77 | SpeI, PstI | 122 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_B 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGTTTGTGAACCAACACCTGTG | 54 bp | 57.98 | XbaI | 149 bp | Potential dimer formation! XbaI+RBS |
| H_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA | 48 bp | 55.10 | SpeI, PstI | 149 bp | Stop codons are removed to enable fusion with export signal. |
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1404 bp | |
| RcsC 3'-2 | GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC | 34 bp | 59.63 | NdeI | 1404 bp | BioBrick standard spacer was added to the 5' side of the NdeI cutting site. |
| [http://partsregistry.org/Part:BBa_G00100 VF2] | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| [http://partsregistry.org/Part:BBa_G00101 VR] | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. |
- [http://www.mdbio.com.tw/pfu.htm pfu primer]
