NYMU Taipei/Primer List
From 2007.igem.org
(Difference between revisions)
Lihsiangyen (Talk | contribs) |
Lihsiangyen (Talk | contribs) |
||
| Line 16: | Line 16: | ||
|- | |- | ||
|M_INS_A 5' | |M_INS_A 5' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTAGATCAGTGCTG</font> |
|54 bp | |54 bp | ||
|56.85 | |56.85 | ||
| Line 33: | Line 33: | ||
|- | |- | ||
|M_INS_B 5' | |M_INS_B 5' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font> |
|55 bp | |55 bp | ||
|60.85 | |60.85 | ||
| Line 51: | Line 51: | ||
|- | |- | ||
|H_INS_A 5' | |H_INS_A 5' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font> |
|54 bp | |54 bp | ||
|62.45 | |62.45 | ||
| Line 68: | Line 68: | ||
|- | |- | ||
|H_INS_B 5' | |H_INS_B 5' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font> |
|54 bp | |54 bp | ||
|57.98 | |57.98 | ||
| Line 86: | Line 86: | ||
|- | |- | ||
|RcsC 5' | |RcsC 5' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GAAATACCTTGCTTCTTTTCG</font> |
| - | | | + | |54 bp |
|55.92 | |55.92 | ||
| - | | | + | |XbaI |
|1404 bp | |1404 bp | ||
| - | | | + | |XbaI+RBS |
|- | |- | ||
|- | |- | ||
Revision as of 17:10, 24 August 2007
Project Description /
Lab Notes /
Part List /
Primer List
| Name | Sequence | Length | Tm (C) | RE site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG | 54 bp | 56.85 | XbaI | 119 bp | Potential dimer formation! XbaI+RBS |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT | 48 bp | 54.74 | SpeI, PstI | 119 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| M_INS_B 5' | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT | 55 bp | 60.85 | XbaI | 149 bp | Potential dimer formation! |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA | 48 bp | 55.86 | SpeI, PstI | 149 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_A 5' | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG | 54 bp | 62.45 | XbaI | 122 bp | Potential dimer formation! XbaI+RBS |
| H_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG | 49 bp | 57.77 | SpeI, PstI | 122 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_B 5' | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG | 54 bp | 57.98 | XbaI | 149 bp | Potential dimer formation! XbaI+RBS |
| H_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA | 48 bp | 55.10 | SpeI, PstI | 149 bp | Stop codons are removed to enable fusion with export signal. |
| RcsC 5' | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG | 54 bp | 55.92 | XbaI | 1404 bp | XbaI+RBS |
| RcsC 3'-2 | GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC | 34 bp | 59.63 | NdeI | 1404 bp | BioBrick standard spacer was added to the 5' side of the NdeI cutting site. |
| [http://partsregistry.org/Part:BBa_G00100 VF2] | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| [http://partsregistry.org/Part:BBa_G00101 VR] | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. |
- [http://www.mdbio.com.tw/pfu.htm pfu primer]
