NYMU Taipei/Lab Notes/2007 8 29
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| - | *Plasmid extraction | + | *Plasmid extraction for [pOmpC+(RBS+EYFP)] |
| - | **pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance) | + | **pOmpC+(RBS+EYFP) 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance) |
**Extract from 1.5ml LB medium to 20ul EB buffer | **Extract from 1.5ml LB medium to 20ul EB buffer | ||
**Stored in -20°C | **Stored in -20°C | ||
| Line 7: | Line 7: | ||
**Emition: 527nm | **Emition: 527nm | ||
*Liquid culture for MGC clones | *Liquid culture for MGC clones | ||
| - | ** INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse) for insulin project | + | ** INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project |
| - | ** PUM1, AKAP1 for m.t. project | + | ** PUM1 (human), AKAP1 (human) for m.t. project |
| + | ---- | ||
| + | ==Who attend?== | ||
| + | * 立翔 | ||
Latest revision as of 07:19, 30 August 2007
- Plasmid extraction for [pOmpC+(RBS+EYFP)]
- pOmpC+(RBS+EYFP) 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
- Extract from 1.5ml LB medium to 20ul EB buffer
- Stored in -20°C
- EYFP spectral characteristics:
- Excitation: 514nm
- Emition: 527nm
- Liquid culture for MGC clones
- INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project
- PUM1 (human), AKAP1 (human) for m.t. project
Who attend?
- 立翔
