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| - | == August 21st, 2007 ==
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| - | Start Time: 11:00 am
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| - | • Charles showed me how to use the registry properly so if anyone needs to know how to use the registry properly just let me know.
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| - | o We searched for super parts which is more like a couple of parts ligated together so that it makes our job a hell lot easier
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| - | o So we chose 7 parts to be transformed from the neural network MODIFIED model
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| - | • Part timer Muhammad Talal Latif showed up and I instructed him on how to do transformation properly
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| - | • So I used 20 l of TE buffer to extract:
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| - | o J 37033: 8F, Plate:3 (Amp Res)
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| - | o S 01640: 3L, Plate:3 (Amp Res)
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| - | • Both of them were from the iGEM 2007 Kit Plates
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| - | • We stored the plasmids in the –20C freezer labeled in red DNA remaining
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| - | • Added 4 l of J37033 and S01640 to separate competent cell (DH5Z1) eppendorf tubes from the –80C freezer
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| - | • So now we are following the Transformation steps from the guidelines posted
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| - | • We used two AMP plates made from before
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| - | • We also made 8 AMP plates where 4 is going to be used today for the other four parts which we also transformed:
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| - | o I 13507: (Used form 2005 stock) (AMP Res)
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| - | o S 01003: 20O, Plate:1 (AMP Res)
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| - | o J 23100: 21E, Plate: 3 (AMP Res)
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| - | o S 01414: 22O, Plate: 1 (AMP Res)
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| - | • We also made 3 AMP and KAN plates where 1 is going to be used:
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| - | o F 1610: 1B, Plate: 2, (AMP and KAN Resistant)
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| - | • CONFUSION: While making the AMP and KAN plates we used AMP of 100mg/ml whereas KAN was 30mg/ml. So even though KAN was of different concentration we used the same quantity.
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| - | o CHARLES AND ANDY GIVE US SOME INSIGHT ON TO THIS TOPIC
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| - | • So we left all 7 plates for overnight incubation and hopefully we see some results tomorrow
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| - | • Everybody please welcome SeungMe (part time) who came in and helped me finish the work today and of course Charles
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