NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 15: Line 15:
!Description
!Description
|-
|-
-
|M_INS_A 5'
+
|M_INS_A F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTAGATCAGTGCTG</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTAGATCAGTGCTG</font>
|54 bp
|54 bp
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|-
|-
|-
|-
-
|M_INS_A 3'
+
|M_INS_A R
-
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
|48 bp
|48 bp
|54.74
|54.74
|SpeI, PstI
|SpeI, PstI
|119 bp
|119 bp
-
|Potential dimer formation! Stop codons are removed to enable fusion with export signal.
+
|Potential dimer formation!
|-
|-
-
|M_INS_B 5'
+
|M_INS_B F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font>
|55 bp
|55 bp
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|-
|-
|-
|-
-
|M_INS_B 3'
+
|M_INS_B R
-
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GGACATGGGTGTGTAGAAGA</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GGACATGGGTGTGTAGAAGA</font>
|48 bp
|48 bp
|55.86
|55.86
|SpeI, PstI
|SpeI, PstI
|149 bp
|149 bp
-
|Potential dimer formation! Stop codons are removed to enable fusion with export signal.
+
|Potential dimer formation!
|-
|-
|-
|-
-
|H_INS_A 5'
+
|H_INS_A F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
|54 bp
|54 bp
Line 59: Line 59:
|-
|-
|-
|-
-
|H_INS_A 3'
+
|H_INS_A R'
-
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GTTGCAGTAGTTCTCCAGCTG</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCTG</font>
|49 bp
|49 bp
|57.77
|57.77
|SpeI, PstI
|SpeI, PstI
|122 bp
|122 bp
-
|Potential dimer formation! Stop codons are removed to enable fusion with export signal.
+
|Potential dimer formation!
|-
|-
-
|H_INS_B 5'
+
|H_INS_B F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font>
|54 bp
|54 bp
Line 76: Line 76:
|-
|-
|-
|-
-
|H_INS_B 3'
+
|H_INS_B R
-
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GGTCTTGGGTGTGTAGAAGA</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GGTCTTGGGTGTGTAGAAGA</font>
|48 bp
|48 bp
|55.10
|55.10
|SpeI, PstI
|SpeI, PstI
|149 bp
|149 bp
-
|Stop codons are removed to enable fusion with export signal.
+
|
|-
|-
|-
|-
-
|RcsC 5'
+
|RcsC F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GAAATACCTTGCTTCTTTTCG</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GAAATACCTTGCTTCTTTTCG</font>
|54 bp
|54 bp
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|-
|-
|-
|-
-
|RcsC 3'-2
+
|RcsC R
|<font color="green">GTTTCTTCCATATG</font><font color="red">CAACGACTCTTCCATCTTCAC</font>
|<font color="green">GTTTCTTCCATATG</font><font color="red">CAACGACTCTTCCATCTTCAC</font>
|34 bp
|34 bp
Line 119: Line 119:
|
|
|Reverse sequencing primer.
|Reverse sequencing primer.
 +
|-
 +
|-M_IDE F
 +
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">CGGAACGGGCTCGTGTGGCT</font>
 +
|54 bp
 +
|
 +
|XbaI
 +
| bp
 +
|Potential dimer formation! XbaI+RBS
 +
|-
 +
|-
 +
|M_IDE R
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GAGTTTTGCAGCCATGAAGT</font>
 +
|48 bp
 +
|54.74
 +
|SpeI, PstI
 +
| bp
 +
|Potential dimer formation!
 +
|-
 +
|-H_IGFBP3 F
 +
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">CAGCGGGCGCGACCCACGCT</font>
 +
|54 bp
 +
|
 +
|XbaI
 +
| bp
 +
|Potential dimer formation! XbaI+RBS
 +
|-
 +
|-
 +
|H_IGFBP3 R
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">CTTGCTCTGCATGCTGTAGC</font>
 +
|48 bp
 +
|54.74
 +
|SpeI, PstI
 +
| bp
 +
|Potential dimer formation!
 +
|-
 +
|-H_IGFBP7 F
 +
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GAGCGGCCGTCGCTGCGCGC</font>
 +
|54 bp
 +
|
 +
|XbaI
 +
| bp
 +
|Potential dimer formation! XbaI+RBS
 +
|-
 +
|-
 +
|H_IGFBP7 R
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">TAGCTCGGCACCTTCACCTT</font>
 +
|48 bp
 +
|54.74
 +
|SpeI, PstI
 +
| bp
 +
|Potential dimer formation!
|-
|-
|}
|}
* [http://www.mdbio.com.tw/pfu.htm pfu primer]
* [http://www.mdbio.com.tw/pfu.htm pfu primer]

Revision as of 09:15, 31 August 2007

Project Description / Lab Notes / Part List / Primer List

Name Sequence Length Tm (C) RE site Target length Description
M_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG 54 bp 56.85 XbaI 119 bp Potential dimer formation! XbaI+RBS
M_INS_A R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 119 bp Potential dimer formation!
M_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT 55 bp 60.85 XbaI 149 bp Potential dimer formation!
M_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 149 bp Potential dimer formation!
H_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG 54 bp 62.45 XbaI 122 bp Potential dimer formation! XbaI+RBS
H_INS_A R' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 122 bp Potential dimer formation!
H_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG 54 bp 57.98 XbaI 149 bp Potential dimer formation! XbaI+RBS
H_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 149 bp
RcsC F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG 54 bp 55.92 XbaI 1404 bp XbaI+RBS
RcsC R GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC 34 bp 59.63 NdeI 1404 bp BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2] tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR] attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCGGAACGGGCTCGTGTGGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
M_IDE R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP3 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP7 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
  • [http://www.mdbio.com.tw/pfu.htm pfu primer]
Retrieved from "http://2007.igem.org/wiki/index.php/NYMU_Taipei/Primer_List"