Primers Design
From 2007.igem.org
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We have design two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc: | We have design two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc: | ||
| - | + | [[Image:oligo.jpg]] | |
2X ORE – CYC1 Hind III ORE Fw | 2X ORE – CYC1 Hind III ORE Fw | ||
5’-CCC [[AAGCTT]] TAGTCAATAATGGGAGCTGG-3’ | 5’-CCC [[AAGCTT]] TAGTCAATAATGGGAGCTGG-3’ | ||
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2X ORE – CYC1 BamHI Rev | 2X ORE – CYC1 BamHI Rev | ||
5’-CGC [[GGATCC]] GTGCGTTTGTGGCGCCCTTA-3’ | 5’-CGC [[GGATCC]] GTGCGTTTGTGGCGCCCTTA-3’ | ||
| - | Tm 68°C | + | Tm 68°C |
1X ORE – CYC1 Hind III ORE Fw | 1X ORE – CYC1 Hind III ORE Fw | ||
Revision as of 09:43, 10 September 2007
We have design two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc:
2X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT TAGTCAATAATGGGAGCTGG-3’ Tm 62°C
2X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’ Tm 68°C
1X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT GGCTCTACCGAGCTGGTAGA-3’
1X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’
LUCIFERASE BamH1 Fv 5’- CGC GGATCC ATGGAAGACGCCAAAAACATAA-3’ Tm 60°C
LUCIFERASE SacI Rv 5’- CGC GAGCTC TTAAAGCTTCTTTCCGCCCTT-3’ Tm 60°C
