Vector choice
From 2007.igem.org
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| - | Our finally vector will be YCplac22.This is a centromeric plasmid, | + | Our finally vector will be YCplac22. |
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| + | This is a YC-type (centromeric) shuttle vector. | ||
| + | |||
| + | Vector Information: | ||
| + | |||
| + | Vector type: plasmid | ||
| + | |||
| + | Size (kb): 4.854 | ||
| + | |||
| + | Construct size (kb): 4.854 | ||
| + | |||
| + | Construction: pUC19 | ||
| + | |||
| + | Markers: TRP1, ampR | ||
| + | |||
| + | Features: insert detection: lacZ' | ||
| + | |||
| + | marker(s): TRP1, ampR | ||
| + | |||
| + | promoter: lac | ||
| + | |||
| + | replicon: ARS1, pMB1 | ||
| + | |||
| + | MCS: HindIII...EcoRI | ||
| + | |||
| + | centromere: CEN4 | ||
| + | |||
[[Image:YCplac22_map.jpg]] | [[Image:YCplac22_map.jpg]] | ||
Revision as of 20:01, 10 September 2007
Our finally vector will be YCplac22.
This is a YC-type (centromeric) shuttle vector.
Vector Information:
Vector type: plasmid
Size (kb): 4.854
Construct size (kb): 4.854
Construction: pUC19
Markers: TRP1, ampR
Features: insert detection: lacZ'
marker(s): TRP1, ampR
promoter: lac
replicon: ARS1, pMB1
MCS: HindIII...EcoRI
centromere: CEN4
Selected features
lac_promoter 142 - 171
M13_pUC_rev_primer 185 - 207
M13_reverse_primer 206 - 224
M13_forward20_primer 306 - 290
M13_pUC_fwd_primer 321 - 299
CEN4 504 - 1356
Orf frame 2 2740 - 2066
TRP1 2740 - 2066
AmpR_promoter 2983 - 3011
Orf frame 2 3053 - 3913
Ampicillin 3053 - 3913
pBR322_origin 4068 - 4687
Unique restriction sites
HindIII 233
PstI 249
SalI 251
XbaI 257
BamHI 263
SmaI 270
KpnI 276
SacI 282
EcoRI 284
NarI 446
SpeI 550
NheI 1801
BglII 1986
EcoRV 2455
PmlI 2780
AatII 2921
