Vector choice
From 2007.igem.org
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| - | Our finally vector will be YCplac22. | + | Our finally vector will be YCplac22,an YC-type (centromeric) shuttle vector. |
| - | + | '''Vector Information''': | |
| - | Vector | + | '''Vector type''': plasmid |
| - | + | '''Size (kb): ''' 4.854 | |
| - | + | '''Construct size (kb): ''' 4.854 | |
| - | + | '''Construction:''' pUC19 | |
| - | + | '''Markers:''' TRP1, ampR | |
| - | + | '''Features:''' insert detection: lacZ' | |
| - | + | '''marker(s):''' TRP1, ampR | |
| - | + | '''promoter:''' lac | |
| - | + | '''replicon:''' ARS1, pMB1 | |
| - | + | '''MCS:''' HindIII...EcoRI | |
| - | + | '''centromere:''' CEN4 | |
| - | + | ||
| - | centromere: CEN4 | + | |
[[Image:YCplac22_map.jpg]] | [[Image:YCplac22_map.jpg]] | ||
| - | Selected features | + | '''Selected features''' |
| - | lac_promoter | + | lac_promoter 142 - 171 |
| - | M13_pUC_rev_primer | + | M13_pUC_rev_primer 185 - 207 |
| - | M13_reverse_primer | + | M13_reverse_primer 206 - 224 |
| - | M13_forward20_primer 306 - 290 | + | M13_forward20_primer 306 - 290 |
| - | M13_pUC_fwd_primer | + | M13_pUC_fwd_primer 321 - 299 |
| - | CEN4 | + | CEN4 504 - 1356 |
| - | Orf frame 2 | + | Orf frame 2 2740 - 2066 |
| - | TRP1 | + | TRP1 2740 - 2066 |
| - | AmpR_promoter | + | AmpR_promoter 2983 - 3011 |
| - | Orf frame 2 | + | Orf frame 2 3053 - 3913 |
| - | Ampicillin | + | Ampicillin 3053 - 3913 |
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| - | + | pBR322_origin 4068 - 4687 | |
Revision as of 20:05, 10 September 2007
Our finally vector will be YCplac22,an YC-type (centromeric) shuttle vector.
Vector Information:
Vector type: plasmid
Size (kb): 4.854
Construct size (kb): 4.854
Construction: pUC19
Markers: TRP1, ampR
Features: insert detection: lacZ'
marker(s): TRP1, ampR
promoter: lac
replicon: ARS1, pMB1
MCS: HindIII...EcoRI
centromere: CEN4
Selected features
lac_promoter 142 - 171
M13_pUC_rev_primer 185 - 207
M13_reverse_primer 206 - 224
M13_forward20_primer 306 - 290
M13_pUC_fwd_primer 321 - 299
CEN4 504 - 1356
Orf frame 2 2740 - 2066
TRP1 2740 - 2066
AmpR_promoter 2983 - 3011
Orf frame 2 3053 - 3913
Ampicillin 3053 - 3913
pBR322_origin 4068 - 4687
