NYMU Taipei/Lab Notes/2007 9 13

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** We tested our PCR product (5uL) from different annealing temperature via GEL
** We tested our PCR product (5uL) from different annealing temperature via GEL
** From imageJ comparison, the concentration of product rise with temperature before 60 degree
** From imageJ comparison, the concentration of product rise with temperature before 60 degree
-
[[Image:20070913-INS A GEL.jpg]]
+
*** GEL (left to right: 49.8, 52.3, 55.5, 58.7, 60.9 degree)
 +
*** [[Image:20070913-INS A GEL.jpg]]
 +
*** imageJ
 +
*** [[Image:20070913-INS A imageJ.jpg]]
 +
*** marker (100 bp, 40ng/10uL = 4ng/uL)
 +
*** the best temperature should be around 55.5 degree
 +
* PCR product concentration summary
 +
{| style="color:green;background-color:#ffffcc;" | border=1 | align=center
 +
!name
 +
!product
 +
!dulition
 +
!A260
 +
!A260/A280
 +
!A260/A230
 +
|-
 +
|9/5 海威
 +
|INS_A #1 (A1)
 +
|6uL + 594uL ddH2O
 +
|0.049
 +
|1.27
 +
|0.47
 +
|-
 +
|9/5 海威
 +
|INS_A #2 (A2)
 +
|6uL + 594uL ddH2O
 +
|0.082
 +
|1.32
 +
|0.51
 +
|-
 +
|9/5 海威
 +
|INS_B #1 (B1)
 +
|6uL + 594uL ddH2O
 +
|0.075
 +
|1.29
 +
|0.48
 +
|-
 +
|9/5 海威
 +
|INS_B #2 (B2)
 +
|6uL + 594uL ddH2O
 +
|0.081
 +
|1.36
 +
|0.52
 +
|-
 +
|9/12 德銘
 +
|INS_A #1 (A1, 49.8)
 +
|6uL + 594uL ddH2O
 +
|0.076
 +
|1.33
 +
|0.52
 +
|-
 +
|9/12 德銘
 +
|INS_A #2 (A2, 52.3)
 +
|6uL + 594uL ddH2O
 +
|0.077
 +
|1.33
 +
|0.52
 +
|-
 +
|9/12 德銘
 +
|INS_A #3 (A3, 55.5)
 +
|6uL + 594uL ddH2O
 +
|0.083
 +
|1.29
 +
|0.56
 +
|-
 +
|9/12 德銘
 +
|INS_A #4 (A4, 58.7)
 +
|6uL + 594uL ddH2O
 +
|0.077
 +
|1.33
 +
|0.52
 +
|-
 +
|9/12 德銘
 +
|INS_A #5 (A1, 60.9)
 +
|6uL + 594uL ddH2O
 +
|0.075
 +
|1.33
 +
|0.52
 +
|-
 +
|9/8 惇茹
 +
|INS_A #1
 +
|4uL + 196uL ddH2O
 +
|0.022
 +
|22
 +
|n/a
 +
|-
 +
|9/8 惇茹
 +
|INS_A #1
 +
|10uL + 190uL ddH2O
 +
|0.056
 +
|12
 +
|n/a
 +
|}
 +
----
 +
* task for the next day
 +
** GEL preparation for INS_A and INS_B

Latest revision as of 13:17, 13 September 2007

  • PCR issue
    • We tested our PCR product (5uL) from different annealing temperature via GEL
    • From imageJ comparison, the concentration of product rise with temperature before 60 degree
      • GEL (left to right: 49.8, 52.3, 55.5, 58.7, 60.9 degree)
      • 20070913-INS A GEL.jpg
      • imageJ
      • 20070913-INS A imageJ.jpg
      • marker (100 bp, 40ng/10uL = 4ng/uL)
      • the best temperature should be around 55.5 degree
  • PCR product concentration summary
name product dulition A260 A260/A280 A260/A230
9/5 海威 INS_A #1 (A1) 6uL + 594uL ddH2O 0.049 1.27 0.47
9/5 海威 INS_A #2 (A2) 6uL + 594uL ddH2O 0.082 1.32 0.51
9/5 海威 INS_B #1 (B1) 6uL + 594uL ddH2O 0.075 1.29 0.48
9/5 海威 INS_B #2 (B2) 6uL + 594uL ddH2O 0.081 1.36 0.52
9/12 德銘 INS_A #1 (A1, 49.8) 6uL + 594uL ddH2O 0.076 1.33 0.52
9/12 德銘 INS_A #2 (A2, 52.3) 6uL + 594uL ddH2O 0.077 1.33 0.52
9/12 德銘 INS_A #3 (A3, 55.5) 6uL + 594uL ddH2O 0.083 1.29 0.56
9/12 德銘 INS_A #4 (A4, 58.7) 6uL + 594uL ddH2O 0.077 1.33 0.52
9/12 德銘 INS_A #5 (A1, 60.9) 6uL + 594uL ddH2O 0.075 1.33 0.52
9/8 惇茹 INS_A #1 4uL + 196uL ddH2O 0.022 22 n/a
9/8 惇茹 INS_A #1 10uL + 190uL ddH2O 0.056 12 n/a
  • task for the next day
    • GEL preparation for INS_A and INS_B
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