Week 11

From 2007.igem.org

(Difference between revisions)
Line 42: Line 42:
-[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1;
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* '''Testing our devices'''
 
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We take 5 ml of LB culture ground in which we put a colony of bacteria which contain Ptet-LacI Plac-cI-LacY-GFP plasmid.
 
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We examine a drop of it using our fluorescence microscopy setup. In these conditions, the bacteria don’t beam fluorescence.
 
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Starting from this moment, we put into the culture ground 1 mM of IPTG every 30 minutes until it is 4 millimolar, checking each time any eventual fluorescence, with negative results. After diluting 1 ml of the previous one with 4 ml of LB, we add IPTG until 1 ml of our new solution is 10 mM, then 20 mM and finally 40 mM. Despite that, no fluorescence is visible.
 
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Every time we checked for fluorescence, we used not only the photo camera but also the photomultiplier; every measurement showed a 0.16-0.18 volts offset, despite the bacteria being non-fluorescent: at least, we regulated that offset!
 

Revision as of 12:19, 14 September 2007

09/10/07
  • Miniprep for

-[http://partsregistry.org/Part:BBa_I763027 I763027];

-[http://partsregistry.org/Part:BBa_I763028 I763028];

-[http://partsregistry.org/Part:BBa_I763019 I763019];

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_P0412 P0412];

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_S03520 S03520];

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_S0100 S0100];

Digestion for:

-[http://partsregistry.org/Part:BBa_I763027 I763027] with Xba/Spe;

-[http://partsregistry.org/Part:BBa_I763028 I763028] with Spe/Pst1 and with Xba/Spe;

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe;

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_P0412 P0412] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_S03520 S03520] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_S0100 S0100] with Eco/Spe;

  • Band extraction from gel for all digestion;
  • We have problems with [http://partsregistry.org/Part:BBa_J52034 J52034] and with [http://partsregistry.org/Part:BBa_I763019 I763019].
  • Ligations for:

-[http://partsregistry.org/Part:BBa_I763028 I763028] + [http://partsregistry.org/Part:BBa_I763007 I763007]

-[http://partsregistry.org/Part:BBa_S0100 S0100] + [http://partsregistry.org/Part:BBa_J04431 J04431], because we want to understand if LacI operates well;

-[http://partsregistry.org/Part:BBa_J06550 J06550] + [http://partsregistry.org/Part:BBa_J04631 J04631], with this ligation we want to understand if it operates well, because it doesn't leak.

09/11/07
  • Digestion for:

-[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1;


09/12/07



09/13/07



09/14/07



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