NYMU Taipei/phptest
From 2007.igem.org
(Difference between revisions)
| Line 17: | Line 17: | ||
<form name=form1> | <form name=form1> | ||
| - | <INPUT type="text" name="insert_size" > (bp) | + | <INPUT type="text" name="insert_size" > size (bp) of insert part (SI)<BR> |
| - | <INPUT type="text" name="vector_size" > (bp) | + | <INPUT type="text" name="vector_size" > size (bp) of vector part (SV)<BR> |
| - | <INPUT type="text" name="I" > (ug/uL) | + | <INPUT type="text" name="I" > concentration (ug/uL) of insert (CI)<BR> |
| - | <INPUT type="text" name="V" > (ug/uL) | + | <INPUT type="text" name="V" > concentration (ug/uL) of vector (CV)<BR> |
<INPUT type="text" name="X" > volume (uL) of insert for gel separation (VI)<BR> | <INPUT type="text" name="X" > volume (uL) of insert for gel separation (VI)<BR> | ||
<INPUT type="text" name="L" > volume (uL) of insert for ligation (L)<BR> | <INPUT type="text" name="L" > volume (uL) of insert for ligation (L)<BR> | ||
| Line 38: | Line 38: | ||
---- | ---- | ||
* formula | * formula | ||
| - | ** (1) 300 * SI * 3 * 10 / SV = CI * X * L /50 | + | ** (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * X * L /50 |
** (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV | ** (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV | ||
** VV (uL) is volume of vector | ** VV (uL) is volume of vector | ||
** CV (ng/uL) is concentration of vector | ** CV (ng/uL) is concentration of vector | ||
Revision as of 07:00, 16 September 2007
- total digestion volume is 20 uL (typical)
- enzyme 1 and enzyme 2 (1uL * 2)
- 10X buffer (2uL)
- BSA (2uL)
- plasmid + ddH2O (14uL)
- total gel separation volume is 50 uL
- vector weight is 300 ng
- formula
- (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * X * L /50
- (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV
- VV (uL) is volume of vector
- CV (ng/uL) is concentration of vector
