NYMU Taipei/phptest

(Difference between revisions)

Revision as of 07:01, 16 September 2007

total digestion volume is 20 uL (typical)
- enzyme 1 and enzyme 2 (1uL * 2)
- 10X buffer (2uL)
- BSA (2uL)
- plasmid + ddH2O (14uL)

formula
- (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * X * L /50
- (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV
- VV (uL) is volume of vector
- CV (ng/uL) is concentration of vector

@@ Line 4: / Line 4: @@
 <script language="javascript">
-function CheckId(v1,v2){
+function CheckId(SI,SV){
-var a = v1;
+var SI = SI;
-var b = v2;
+var SV = SV;
 //document.write("insert is v1, and vector is v2");
-alert("insert size is " + a + " \nand vector size is " + b);
+alert("insert size is " + SI + " \nand vector size is " + SV);
 }
@@ Line 17: / Line 17: @@
 <form name=form1>
-     <INPUT type="text" name="insert_size" >  size (bp) of insert part (SI)<BR>
+     <INPUT type="text" name="SI" >  size (bp) of insert part (SI)<BR>
-     <INPUT type="text" name="vector_size" >  size (bp) of vector part (SV)<BR>
+     <INPUT type="text" name="SV" >  size (bp) of vector part (SV)<BR>
      <INPUT type="text" name="I" > concentration (ug/uL) of insert (CI)<BR>
      <INPUT type="text" name="V" > concentration (ug/uL) of vector (CV)<BR>
      <INPUT type="text" name="X" > volume (uL) of insert for gel separation (VI)<BR>
      <INPUT type="text" name="L" > volume (uL) of insert for  ligation (L)<BR>
-     <input type=button value="送出" Onclick="CheckId(insert_size.value, vector_size.value);">
+     <input type=button value="送出" Onclick="CheckId(SI.value, SV.value);">
 </form>
 </body>