NYMU Taipei/phptest
From 2007.igem.org
(Difference between revisions)
| Line 22: | Line 22: | ||
<INPUT type="text" name="CV" > concentration (ug/uL) of vector (CV)<BR> | <INPUT type="text" name="CV" > concentration (ug/uL) of vector (CV)<BR> | ||
<INPUT type="text" name="VI" > volume (uL) of insert for gel separation (VI)<BR> | <INPUT type="text" name="VI" > volume (uL) of insert for gel separation (VI)<BR> | ||
| - | <INPUT type="text" name="L" > volume (uL) of insert for | + | <INPUT type="text" name="L" > volume (uL) of insert for ligation (L)<BR> |
<input type=button value="送出" Onclick="CheckId(SI.value, SV.value, CI.value, CV.value, VI.value, L.value);"> | <input type=button value="送出" Onclick="CheckId(SI.value, SV.value, CI.value, CV.value, VI.value, L.value);"> | ||
</form> | </form> | ||
| Line 40: | Line 40: | ||
** 10X buffer (1uL) | ** 10X buffer (1uL) | ||
** ligase (1uL) | ** ligase (1uL) | ||
| - | ** VV + | + | ** VV + L + water (8uL) |
---- | ---- | ||
* formula | * formula | ||
| - | ** (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * | + | ** (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * VI * L /50 |
** (2) 300 ng = CV (ug/uL) * VV (uL) * 1000, VV = 0.3/CV | ** (2) 300 ng = CV (ug/uL) * VV (uL) * 1000, VV = 0.3/CV | ||
** VV (uL) is volume of vector | ** VV (uL) is volume of vector | ||
** CV (ng/uL) is concentration of vector | ** CV (ng/uL) is concentration of vector | ||
Revision as of 07:14, 16 September 2007
- total digestion volume is 20 uL (typical)
- enzyme 1 and enzyme 2 (1uL * 2)
- 10X buffer (2uL)
- BSA (2uL)
- plasmid + ddH2O (14uL = VI + water, 1 <= VI <= 14)
- total gel separation volume is 50 uL
- total ligation volume is 10uL
- vector weight is 300 ng
- 10X buffer (1uL)
- ligase (1uL)
- VV + L + water (8uL)
- formula
- (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * VI * L /50
- (2) 300 ng = CV (ug/uL) * VV (uL) * 1000, VV = 0.3/CV
- VV (uL) is volume of vector
- CV (ng/uL) is concentration of vector
