NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 249: Line 249:
|TAT signal peptide
|TAT signal peptide
|<font color="lightseagreen">atggacaaattcgacgct</font><font color="black">aatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctg</font><font color=purple>ccgacccctgcgtttgca</font>
|<font color="lightseagreen">atggacaaattcgacgct</font><font color="black">aatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctg</font><font color=purple>ccgacccctgcgtttgca</font>
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|91 bp
+
|90 bp
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|
|
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|TAT_FP
|TAT_FP
|<font color="green">AAG TCTAGA aaagaggagaaa ACTAG </font><font color="red">ATGgacaaattcgacgct</font>
|<font color="green">AAG TCTAGA aaagaggagaaa ACTAG </font><font color="red">ATGgacaaattcgacgct</font>
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|45 bp
+
|44 bp
|56.57
|56.57
|XbaI
|XbaI
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|91 bp
+
|90 bp
|TAT signal peptide forward primer
|TAT signal peptide forward primer
 +
|-
 +
|INS_A
 +
|<font color="orange">GGCATTGTGGAACAATGC</font><font color="black">TGTACCAGCATCTGCTCCCTCTACCAGCTGGAG</font><font color=>AACTACTGCAACTAGTAG</font>
 +
|69 bp
 +
|
 +
|
 +
|
 +
|human insulin A chain
|-
|-
|}
|}
* [http://www.mdbio.com.tw/pfu.htm pfu primer]
* [http://www.mdbio.com.tw/pfu.htm pfu primer]
* [http://partsregistry.org/Help:Contents Biobrick Help]
* [http://partsregistry.org/Help:Contents Biobrick Help]

Revision as of 16:03, 19 September 2007

Project Description / Lab Notes / Part List / Primer List

Name Sequence Length Tm (C) RE site Target length Description
M_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG 54 bp 56.85 XbaI 119 bp Potential dimer formation! XbaI+RBS
M_INS_A R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 119 bp Potential dimer formation!
M_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT 55 bp 60.85 XbaI 149 bp Potential dimer formation!
M_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 149 bp Potential dimer formation!
TAT F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG 56 bp 59.92 XbaI bp XbaI+RBS
TAT F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG 108 bp 59.92 XbaI bp XbaI+RBS
H_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG 54 bp 62.45 XbaI 122 bp Potential dimer formation! XbaI+RBS
H_INS_A TAT F CGCTTGCGCCGCAGTCGCACGGCATTGTGGAACAATGCTG bp bp
TAT H_INS_A R CAGCATTGTTCCACAATGCCCGCTTGCGCCGC 32 bp 59.26 bp Potential dimer formation!
H_INS_A R' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 122 bp Potential dimer formation!
H_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG 54 bp 57.98 XbaI 149 bp Potential dimer formation! XbaI+RBS
H_INS_B TAT F CGCTTGCGCCGCAGTCGCACTTTGTGAACCAACACCTGTG bp bp
TAT H_INS_B R CACAGGTGTTGGTTCACAAACGCTTGCGCCGC bp bp
H_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 149 bp
RcsC F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG 54 bp 55.92 XbaI 1404 bp XbaI+RBS
RcsC R GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC 34 bp 59.63 NdeI 1404 bp BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2] tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR] attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
M_IDE TAT F CGCTTGCGCCGCAGTCGCACCGGAACGGGCTCGTGTGGCT 40 bp bp Potential dimer formation!
TAT M_IDE R AGCCACACGAGCCCGTTCCGCGCTTGCGCCGC bp bp
M_IDE R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP3 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP3 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP7 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP7 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
OmpRBS F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACATTTTGAAACATCTATAGCG bp bp Primer dimer
OmpRBS R GTTTCTTCCTGCAGCGGCCGCTACTAGTAACGAATATGATACTAAAACTTTTAAGAT bp bp Primer dimer
TAT signal peptide atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgca 90 bp TAT signal peptide for transport protein expressed in E.coli
TAT_FP AAG TCTAGA aaagaggagaaa ACTAG ATGgacaaattcgacgct 44 bp 56.57 XbaI 90 bp TAT signal peptide forward primer
INS_A GGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAACTAGTAG 69 bp human insulin A chain
  • [http://www.mdbio.com.tw/pfu.htm pfu primer]
  • [http://partsregistry.org/Help:Contents Biobrick Help]
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