From 2007.igem.org

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	|90 bp		|90 bp
	|TAT signal peptide forward primer		|TAT signal peptide forward primer
		+	|-
		+	|TAT_RP
		+	|<font color="orange">GCATTGTTCCACAATGCC </font><font color="purple">tgcaaacgcaggggtcgg</font>
		+	|bp
		+	|
		+	|
		+	|90 bp
		+	|TAT signal peptide reverse primer
	|-		|-
	|INS_A		|INS_A

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Revision as of 16:17, 19 September 2007

Project Description / Lab Notes / Part List / Primer List

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Name	Sequence	Length	Tm (C)	RE site	Target length	Description
M_INS_A F	GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG	54 bp	56.85	XbaI	119 bp	Potential dimer formation! XbaI+RBS
M_INS_A R	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT	48 bp	54.74	SpeI, PstI	119 bp	Potential dimer formation!
M_INS_B F	GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT	55 bp	60.85	XbaI	149 bp	Potential dimer formation!
M_INS_B R	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA	48 bp	55.86	SpeI, PstI	149 bp	Potential dimer formation!
TAT F	GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG	56 bp	59.92	XbaI	bp	XbaI+RBS
TAT F	GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG	108 bp	59.92	XbaI	bp	XbaI+RBS
H_INS_A F	GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG	54 bp	62.45	XbaI	122 bp	Potential dimer formation! XbaI+RBS
H_INS_A TAT F	CGCTTGCGCCGCAGTCGCACGGCATTGTGGAACAATGCTG	bp			bp
TAT H_INS_A R	CAGCATTGTTCCACAATGCCCGCTTGCGCCGC	32 bp	59.26		bp	Potential dimer formation!
H_INS_A R'	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG	49 bp	57.77	SpeI, PstI	122 bp	Potential dimer formation!
H_INS_B F	GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG	54 bp	57.98	XbaI	149 bp	Potential dimer formation! XbaI+RBS
H_INS_B TAT F	CGCTTGCGCCGCAGTCGCACTTTGTGAACCAACACCTGTG	bp			bp
TAT H_INS_B R	CACAGGTGTTGGTTCACAAACGCTTGCGCCGC	bp			bp
H_INS_B R	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA	48 bp	55.10	SpeI, PstI	149 bp
RcsC F	GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG	54 bp	55.92	XbaI	1404 bp	XbaI+RBS
RcsC R	GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC	34 bp	59.63	NdeI	1404 bp	BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2]	tgccacctgacgtctaagaa	20 bp	59.44			Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR]	attaccgcctttgagtgagc	20 bp	59.34			Reverse sequencing primer.
M_IDE TAT F	CGCTTGCGCCGCAGTCGCACCGGAACGGGCTCGTGTGGCT	40 bp			bp	Potential dimer formation!
TAT M_IDE R	AGCCACACGAGCCCGTTCCGCGCTTGCGCCGC	bp			bp
M_IDE R	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT	48 bp	54.74	SpeI, PstI	bp	Potential dimer formation!
H_IGFBP3 F	GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT	54 bp		XbaI	bp	Potential dimer formation! XbaI+RBS
H_IGFBP3 R	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC	48 bp	54.74	SpeI, PstI	bp	Potential dimer formation!
H_IGFBP7 F	GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC	54 bp		XbaI	bp	Potential dimer formation! XbaI+RBS
H_IGFBP7 R	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT	48 bp	54.74	SpeI, PstI	bp	Potential dimer formation!
OmpRBS F	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACATTTTGAAACATCTATAGCG	bp			bp	Primer dimer
OmpRBS R	GTTTCTTCCTGCAGCGGCCGCTACTAGTAACGAATATGATACTAAAACTTTTAAGAT	bp			bp	Primer dimer
TAT signal peptide	atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgca	90 bp				TAT signal peptide for transport protein expressed in E.coli
TAT_FP	AAG TCTAGA aaagaggagaaa ACTAG ATGgacaaattcgacgct	44 bp	56.57	XbaI	90 bp	TAT signal peptide forward primer
TAT_RP	GCATTGTTCCACAATGCC tgcaaacgcaggggtcgg	bp			90 bp	TAT signal peptide reverse primer
INS_A	GGCATTGTGGAACAATGC TGTACCAGCATCTGCTCCCTCTACCAGCTGGAG AACTACTGCAAC TAG	63 + 3 bp				human insulin A chain

• [http://www.mdbio.com.tw/pfu.htm pfu primer]
• [http://partsregistry.org/Help:Contents Biobrick Help]