NYMU Taipei/Primer List
From 2007.igem.org
(Difference between revisions)
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|TAT_FP | |TAT_FP | ||
| - | |<font color="black">AAG TCTAGA aaagaggagaaa ACTAG </font><font color="lightseagreen">ATG | + | |<font color="black">AAG TCTAGA aaagaggagaaa ACTAG </font><font color="lightseagreen">ATG gacaaattcgacgc </font> |
| - | |44 bp | + | |44 - 1 bp |
| - | | | + | |55.26 |
|XbaI | |XbaI | ||
|90 bp | |90 bp | ||
| Line 264: | Line 264: | ||
|- | |- | ||
|TAT_RP | |TAT_RP | ||
| - | |<font color="orange">GCATTGTTCCACAATGCC </font><font color="purple"> | + | |<font color="orange">GCATTGTTCCACAATGCC </font><font color="purple">tgcaaacgcaggggt</font> |
| - | |36 bp | + | |36 -3 bp |
| - | | | + | |58.05 |
| - | | | + | |potential dimer (overlap 10) |
|90 bp | |90 bp | ||
|TAT signal peptide reverse primer | |TAT signal peptide reverse primer | ||
| Line 283: | Line 283: | ||
|36 bp | |36 bp | ||
|58.40 | |58.40 | ||
| - | | | + | |potential dimer (overlap 17) |
|63 bp | |63 bp | ||
|INS_A forward primer with TAT | |INS_A forward primer with TAT | ||
| Line 298: | Line 298: | ||
* [http://www.mdbio.com.tw/pfu.htm pfu primer] | * [http://www.mdbio.com.tw/pfu.htm pfu primer] | ||
* [http://partsregistry.org/Help:Contents Biobrick Help] | * [http://partsregistry.org/Help:Contents Biobrick Help] | ||
| + | * Tm is estimated by Amplify 3 | ||
Latest revision as of 10:03, 20 September 2007
Project Description /
Lab Notes /
Part List /
Primer List
| Name | Sequence | Length | Tm (C) | RE site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG | 54 bp | 56.85 | XbaI | 119 bp | Potential dimer formation! XbaI+RBS |
| M_INS_A R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT | 48 bp | 54.74 | SpeI, PstI | 119 bp | Potential dimer formation! |
| M_INS_B F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT | 55 bp | 60.85 | XbaI | 149 bp | Potential dimer formation! |
| M_INS_B R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA | 48 bp | 55.86 | SpeI, PstI | 149 bp | Potential dimer formation! |
| TAT F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG | 56 bp | 59.92 | XbaI | bp | XbaI+RBS |
| TAT F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG | 108 bp | 59.92 | XbaI | bp | XbaI+RBS |
| H_INS_A F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG | 54 bp | 62.45 | XbaI | 122 bp | Potential dimer formation! XbaI+RBS |
| H_INS_A TAT F | CGCTTGCGCCGCAGTCGCACGGCATTGTGGAACAATGCTG | bp | bp | |||
| TAT H_INS_A R | CAGCATTGTTCCACAATGCCCGCTTGCGCCGC | 32 bp | 59.26 | bp | Potential dimer formation! | |
| H_INS_A R' | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG | 49 bp | 57.77 | SpeI, PstI | 122 bp | Potential dimer formation! |
| H_INS_B F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG | 54 bp | 57.98 | XbaI | 149 bp | Potential dimer formation! XbaI+RBS |
| H_INS_B TAT F | CGCTTGCGCCGCAGTCGCACTTTGTGAACCAACACCTGTG | bp | bp | |||
| TAT H_INS_B R | CACAGGTGTTGGTTCACAAACGCTTGCGCCGC | bp | bp | |||
| H_INS_B R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA | 48 bp | 55.10 | SpeI, PstI | 149 bp | |
| RcsC F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG | 54 bp | 55.92 | XbaI | 1404 bp | XbaI+RBS |
| RcsC R | GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC | 34 bp | 59.63 | NdeI | 1404 bp | BioBrick standard spacer was added to the 5' side of the NdeI cutting site. |
| [http://partsregistry.org/Part:BBa_G00100 VF2] | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| [http://partsregistry.org/Part:BBa_G00101 VR] | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. | ||
| M_IDE TAT F | CGCTTGCGCCGCAGTCGCACCGGAACGGGCTCGTGTGGCT | 40 bp | bp | Potential dimer formation! | ||
| TAT M_IDE R | AGCCACACGAGCCCGTTCCGCGCTTGCGCCGC | bp | bp | |||
| M_IDE R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT | 48 bp | 54.74 | SpeI, PstI | bp | Potential dimer formation! |
| H_IGFBP3 F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT | 54 bp | XbaI | bp | Potential dimer formation! XbaI+RBS | |
| H_IGFBP3 R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC | 48 bp | 54.74 | SpeI, PstI | bp | Potential dimer formation! |
| H_IGFBP7 F | GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC | 54 bp | XbaI | bp | Potential dimer formation! XbaI+RBS | |
| H_IGFBP7 R | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT | 48 bp | 54.74 | SpeI, PstI | bp | Potential dimer formation! |
| OmpRBS F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACATTTTGAAACATCTATAGCG | bp | bp | Primer dimer | ||
| OmpRBS R | GTTTCTTCCTGCAGCGGCCGCTACTAGTAACGAATATGATACTAAAACTTTTAAGAT | bp | bp | Primer dimer | ||
| TAT signal peptide | ATG gacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgca | 90 bp | TAT signal peptide for transport protein expressed in E.coli | |||
| TAT_FP | AAG TCTAGA aaagaggagaaa ACTAG ATG gacaaattcgacgc | 44 - 1 bp | 55.26 | XbaI | 90 bp | TAT signal peptide forward primer |
| TAT_RP | GCATTGTTCCACAATGCC tgcaaacgcaggggt | 36 -3 bp | 58.05 | potential dimer (overlap 10) | 90 bp | TAT signal peptide reverse primer |
| INS_A | GGCATTGTGGAACAATGC TGTACCAGCATCTGCTCCCTCTACCAGCTGGAG AACTACTGCAAC TAG | 63 + 3 bp | human insulin A chain | |||
| TAT_INS_A_FP | ccgacccctgcgtttgca GGCATTGTGGAACAATGC | 36 bp | 58.40 | potential dimer (overlap 17) | 63 bp | INS_A forward primer with TAT |
| TAT_INS_A_RP | ctgcag cggccgct actagt a TTA TTA CTAGTTGCAGTAGTT CTCCAG | 42 + 6 bp | 52.05 | SpeI, PstI | 63 bp | INS_A reverse primer with TAT |
- [http://www.mdbio.com.tw/pfu.htm pfu primer]
- [http://partsregistry.org/Help:Contents Biobrick Help]
- Tm is estimated by Amplify 3
