From 2007.igem.org

Revision as of 13:52, 20 September 2007 (view source) Silvia.tamarri2 (Talk | contribs) ← Older edit		Revision as of 13:58, 20 September 2007 (view source) Silvia.tamarri2 (Talk | contribs) Newer edit →
Line 16:		Line 16:
	::'''09/19/07'''		::'''09/19/07'''
	*Miniprep for:		*Miniprep for:
-	-[http://partsregistry.org/Part:BBa_I763027 I763027];	+	-[http://partsregistry.org/Part:BBa_I763028 I763028];
-	-[http://partsregistry.org/Part:BBa_J04031 J04031]J04431;	+	-Ptet-LacI-T-PLac-GFP;
	-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).		-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).
	*Digestion for:		*Digestion for:
-	-I763027 with Eco/Spe;	+	-[http://partsregistry.org/Part:BBa_I763028 I763028] with Eco/Spe;
-	-J04431 with Eco/Spe;	+	-Ptet-LacI-T-PLac-GFP with Eco/Spe;
	-Ptet-LacI-GFP with Eco/Spe;		-Ptet-LacI-GFP with Eco/Spe;
-	-I763007 with Eco/Xba;	+	-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/Xba;
	-Plac-cI-LacY with Eco/Spe1;		-Plac-cI-LacY with Eco/Spe1;

---

Revision as of 13:58, 20 September 2007

09/17/07

• Ligations for:

-[http://partsregistry.org/Part:BBa_I763026 I763026] + -[http://partsregistry.org/Part:BBa_I763019 I763019];

-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763004 I763004];

-[http://partsregistry.org/Part:BBa_I763025 I763025] + [http://partsregistry.org/Part:BBa_J04031 J04031].

• We transform ligations and strake them on plates.

09/18/07

• We inoculate a colony for yesterday ligations in 5ml of LB medium O/N.

09/19/07

• Miniprep for:

-[http://partsregistry.org/Part:BBa_I763028 I763028];

-Ptet-LacI-T-PLac-GFP;

-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).

• Digestion for:

-[http://partsregistry.org/Part:BBa_I763028 I763028] with Eco/Spe;

-Ptet-LacI-T-PLac-GFP with Eco/Spe;

-Ptet-LacI-GFP with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/Xba;

-Plac-cI-LacY with Eco/Spe1;

• Band extraction from gel for all digestion and then we observe:

-I763027, J04431 are died;

-Ptet-LacI-GFP is correct;

-I763007 not found;

-Plac-cI-LacY is correct.

09/20/07

09/21/07

Back