Week 12
From 2007.igem.org
| Line 1: | Line 1: | ||
::'''09/17/07''' | ::'''09/17/07''' | ||
*Ligations for: | *Ligations for: | ||
| - | -[http://partsregistry.org/Part:BBa_I763026 I763026] + | + | -[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763019 I763019]; |
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763004 I763004]; | -[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763004 I763004]; | ||
| Line 33: | Line 33: | ||
*Band extraction from gel for all digestion and then we observe: | *Band extraction from gel for all digestion and then we observe: | ||
| - | - | + | -[http://partsregistry.org/Part:BBa_I763028 I763028], Ptet-LacI-T-PLac-GFP are died; |
-Ptet-LacI-GFP is correct; | -Ptet-LacI-GFP is correct; | ||
| - | -I763007 not found; | + | -[http://partsregistry.org/Part:BBa_I763007 I763007] not found; |
-Plac-cI-LacY is correct. | -Plac-cI-LacY is correct. | ||
Revision as of 14:00, 20 September 2007
- 09/17/07
- Ligations for:
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763019 I763019];
-[http://partsregistry.org/Part:BBa_I763026 I763026] + [http://partsregistry.org/Part:BBa_I763004 I763004];
-[http://partsregistry.org/Part:BBa_I763025 I763025] + [http://partsregistry.org/Part:BBa_J04031 J04031].
- We transform ligations and strake them on plates.
- 09/18/07
- We inoculate a colony for yesterday ligations in 5ml of LB medium O/N.
- 09/19/07
- Miniprep for:
-[http://partsregistry.org/Part:BBa_I763028 I763028];
-Ptet-LacI-T-PLac-GFP;
-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).
- Digestion for:
-[http://partsregistry.org/Part:BBa_I763028 I763028] with Eco/Spe;
-Ptet-LacI-T-PLac-GFP with Eco/Spe;
-Ptet-LacI-GFP with Eco/Spe;
-[http://partsregistry.org/Part:BBa_I763007 I763007] with Eco/Xba;
-Plac-cI-LacY with Eco/Spe1;
- Band extraction from gel for all digestion and then we observe:
-[http://partsregistry.org/Part:BBa_I763028 I763028], Ptet-LacI-T-PLac-GFP are died;
-Ptet-LacI-GFP is correct;
-[http://partsregistry.org/Part:BBa_I763007 I763007] not found;
-Plac-cI-LacY is correct.
- 09/20/07
- 09/21/07
