BerkiGEM2007Present3
From 2007.igem.org
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| - | + | ===The Controller=== | |
The Controller is an integrated genetic circuit, comprised of two plasmids, that directs the initiation and production of the primary systems proteins through its component operons. | The Controller is an integrated genetic circuit, comprised of two plasmids, that directs the initiation and production of the primary systems proteins through its component operons. | ||
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| + | '''Introduction''' | ||
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| + | As with many synthetic biology applications, we wished to be able to control the production of the proteins involved in our system, control the relative amounts of protein produced, and have a large dynamic range between the off and on states of our system. Therefore, we designed our system to accomplish these goals, by enabling the activation of the system through an Iron Promoter, and put our genes under the control of the T7 RNA Polymerase, with T7 promoters of varying strengths. In addition, we placed the primary genes on an Amplifiable BAC, to enable even more dynamic range. | ||
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| + | '''The Two-Plasmid Pir/T7 System''' | ||
| + | Our controller system is composed of two plasmids: a pSC101 Controller Plasmid, and an Amplifiable BAC. | ||
| + | [ Insert Cool Figure Here ] | ||
Revision as of 22:09, 20 September 2007
The Controller
The Controller is an integrated genetic circuit, comprised of two plasmids, that directs the initiation and production of the primary systems proteins through its component operons.
Introduction
As with many synthetic biology applications, we wished to be able to control the production of the proteins involved in our system, control the relative amounts of protein produced, and have a large dynamic range between the off and on states of our system. Therefore, we designed our system to accomplish these goals, by enabling the activation of the system through an Iron Promoter, and put our genes under the control of the T7 RNA Polymerase, with T7 promoters of varying strengths. In addition, we placed the primary genes on an Amplifiable BAC, to enable even more dynamic range.
The Two-Plasmid Pir/T7 System Our controller system is composed of two plasmids: a pSC101 Controller Plasmid, and an Amplifiable BAC. [ Insert Cool Figure Here ]
