Chiba/Project Description

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(Marimo Bacteria)
 
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==Marimo Bacteria==
==Marimo Bacteria==
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We want E.coli to get together (mediate between flagellar) and shape like  
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Our iGEM project is to make ''E.coli'' get together (mediate between flagellar) and shape like Marimo. Marimo is a Japanese translation of Cladophora ball, Lake Ball, or Moss Balls.  
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Marimo. Marimo is a Japanese translation of Cladophora ball, Lake Ball, or Moss Balls.  
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To do this, we coustruct two E coli populations. E coli A send a singal to call E. coli B, and B get together to make a sheprical cluster by way of the flagella coupling. E coli B also contains another curcuit to sense the size of the cluster (the diameter of Marimo) and turn on/ off the sticky-fragella formation. Thus we can control the size of marimo.
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To do this, we coustruct two E coli populations. E coli A send a singal to call E. coli B, and B get together to make a sheprical cluster by way of the flagella coupling. E coli B also contains another circuit to sense the size of the cluster (the diameter of Marimo) and turn on/ off the sticky-fragella formation. Thus we can control the size of marimo.
To sense the diameter of Marimo, we are consindering to use the concentration gradient of signal molecule (AHL).  
To sense the diameter of Marimo, we are consindering to use the concentration gradient of signal molecule (AHL).  
CURRENTLY, WE ARE THINKING THE FOLLOWING CONSTRUCT AS OF TODAY (8/6/2007).
CURRENTLY, WE ARE THINKING THE FOLLOWING CONSTRUCT AS OF TODAY (8/6/2007).
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[[Image:Circuit_chiba.jpg]]
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[[Image:Circuit_chiba2.jpg]]

Latest revision as of 03:26, 24 September 2007

Marimo Bacteria

Our iGEM project is to make E.coli get together (mediate between flagellar) and shape like Marimo. Marimo is a Japanese translation of Cladophora ball, Lake Ball, or Moss Balls.

To do this, we coustruct two E coli populations. E coli A send a singal to call E. coli B, and B get together to make a sheprical cluster by way of the flagella coupling. E coli B also contains another circuit to sense the size of the cluster (the diameter of Marimo) and turn on/ off the sticky-fragella formation. Thus we can control the size of marimo. To sense the diameter of Marimo, we are consindering to use the concentration gradient of signal molecule (AHL).

CURRENTLY, WE ARE THINKING THE FOLLOWING CONSTRUCT AS OF TODAY (8/6/2007).

Circuit chiba.jpg Circuit chiba2.jpg