Boston University
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## A...ose beads and fluorocytometer | ## A...ose beads and fluorocytometer | ||
## Metallo-Antibiotics | ## Metallo-Antibiotics | ||
| - | ## Perhaps we could split our collection of mutants into different groups, measure their absorbances with spectrophotometry, and assume that the sample with the lowest absorbance contains mutants producing more electricity and | + | ## (DB's random idea): Could we take advantage of spectrophotometry? Perhaps we could split our collection of mutants into different groups, measure their absorbances with spectrophotometry, and assume that the sample with the lowest absorbance contains mutants producing more electricity and therefore growing slower. We could then split this sample into different groups and repeat. While there might be some inefficient strains in the successful broth samples, on the whole, the broth might be a good one for use in a fuel cell. Problem: Low absorbance could be due to mutants losing viability. Potential Solution: Let initial sample grow for a while so all mutants unable to grow will die off, all mutants able to grow will thrive, and then perform the screen. |
== Week's (Ambitious) Goals == | == Week's (Ambitious) Goals == | ||
Revision as of 23:10, 29 May 2007
Contents |
About Us
Welcome to the wiki for Boston University's iGEM 2007 team!
Our team consists of David Shi, Rahul Ahuja, Christian Ling, and Danny Bellin, all soon-to-be juniors majoring in Biomedical Engineering at Boston University.
We are advised by [http://www.bu.edu/dbin/bme/faculty/?prof=tgardner Dr. Timothy Gardner], Assistant Professor of Biomedical Engineering, as well as Frank Juhn and Stephen Schneider, students in the [http://www.gardnerlab.bu.edu Gardner Laboratory], where we work. We are grateful to our advisors for their time and support!
We are also grateful to Pfizer for their generous support of our team.
Our Project Plan
Our project is aimed at increasing current production from Shewanella Oneidensis by directed evolution of global transcription factors.
Our plan so far:
- Mutate global transcription factors
- Transform mutated genes into E. coli
- Choose plasmids
- Restriction enzyme digestion
- Ligation
- Transformation into E. coli
- Conjugate E. coli with Shewanella
- Screen/select for increased current production due to mutations. Potential methods:
- A...ose beads and fluorocytometer
- Metallo-Antibiotics
- (DB's random idea): Could we take advantage of spectrophotometry? Perhaps we could split our collection of mutants into different groups, measure their absorbances with spectrophotometry, and assume that the sample with the lowest absorbance contains mutants producing more electricity and therefore growing slower. We could then split this sample into different groups and repeat. While there might be some inefficient strains in the successful broth samples, on the whole, the broth might be a good one for use in a fuel cell. Problem: Low absorbance could be due to mutants losing viability. Potential Solution: Let initial sample grow for a while so all mutants unable to grow will die off, all mutants able to grow will thrive, and then perform the screen.
Week's (Ambitious) Goals
Wednesday 5/30
- Get all protocols
- Identify materials/prepare order
- Design Primers
- Learn about budget/POs
Thursday 5/31
- Do primer order
- Start conjugation practice
- Confirm restriction enzymes, ligases
- Order confirmed/needed materials
- Team Revew Meeting
Friday 6/1
- Evaluate/continue conjugation, practice electroporation for E. coli
- Meeting with Tim: Budgets/protocols, Pfizer/fundraising, iGEM registration, beads
Materials We Need
Primers: Need to Buy
Error-Prone PCR: Need to Buy
Plasmids: ?Need to Buy
Restriction Enzymes: ?Need to Buy
Ligases: ?Need to Buy
Short-Term Status
Lab Orientation: Completed
Design of Primers: Not Completed
Ordering of Primers: Not Completed
Gathering of Protocols: Not Completed
Ordering of Error-Prone PCR Materials: Not completed
