NYMU Taipei/Lab Notes/2007 9 27
From 2007.igem.org
(Difference between revisions)
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* After re-culture in 20-block plate,randomly pick 6 blocks from CinR+HSL+D-term plate | * After re-culture in 20-block plate,randomly pick 6 blocks from CinR+HSL+D-term plate | ||
* PCR check using VF2, VR primers | * PCR check using VF2, VR primers | ||
| + | * PCR total 50 uL, 10 uL for GEL separation | ||
[[Image:NYMU Taipei 20070927 CinR-HSL-D-term plate culture test.jpg]] | [[Image:NYMU Taipei 20070927 CinR-HSL-D-term plate culture test.jpg]] | ||
* sample at each lane | * sample at each lane | ||
** 1Kb ladder | ** 1Kb ladder | ||
| - | ** block #1 | + | ** block #1 (insert band appears) |
| - | ** block #2 | + | ** block #2 (insert band appears, and one other band around 400 bp) |
| - | ** block #3 | + | ** block #3 () |
** block #4 | ** block #4 | ||
** block #5 | ** block #5 | ||
** block #6 | ** block #6 | ||
| - | * expect results | + | * expect results: one band at 1,623 bp is our insert |
| - | + | ** insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163) | |
| + | ** insert = 790 + 730 + 103 = 1,623 | ||
Revision as of 09:10, 27 September 2007
- After re-culture in 20-block plate,randomly pick 6 blocks from CinR+HSL+D-term plate
- PCR check using VF2, VR primers
- PCR total 50 uL, 10 uL for GEL separation
- sample at each lane
- 1Kb ladder
- block #1 (insert band appears)
- block #2 (insert band appears, and one other band around 400 bp)
- block #3 ()
- block #4
- block #5
- block #6
- expect results: one band at 1,623 bp is our insert
- insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163)
- insert = 790 + 730 + 103 = 1,623
