Paris/September 27
From 2007.igem.org
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Eismoustique (Talk | contribs) |
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| Line 18: | Line 18: | ||
| style="background: #ccffcc;" |D56 | | style="background: #ccffcc;" |D56 | ||
|araC/pBad>>RBS-Cre BBinsert | |araC/pBad>>RBS-Cre BBinsert | ||
| - | |L26. | + | |L26.2 araC/pbad>>RBS-Cre in <bbpart>J61002</bbpart> |
|EcoRI | |EcoRI | ||
|Pst1 | |Pst1 | ||
| Line 62: | Line 62: | ||
| style="background: #ccffcc;" |D75 | | style="background: #ccffcc;" |D75 | ||
|AraC-pBAD-RBS-CRE FI | |AraC-pBAD-RBS-CRE FI | ||
| - | |L26. | + | |L26.2 AraC-pBAD-RBS-CRE |
|EcoRI | |EcoRI | ||
|SpeI | |SpeI | ||
Revision as of 15:52, 28 September 2007
Digestions
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D56 | araC/pBad>>RBS-Cre BBinsert | L26.2 araC/pbad>>RBS-Cre in J61002 | EcoRI | Pst1 | araC/pBad>>RBS-Cre BBinsert (for transfer to the low-copy plasmid vector) | ||||
| D66 | KanR BBa_J61025 FV | (MP12.1, MP12.2) BBa_P1003 in pSB1A2 | EcoR | XbaI | KanR BBa_J61025 (for forward introduction of a casette for Wanner K insertion) | ||||
| D72 | pSB2K3 BBvector | MP16 pSB2K3::attP | EcoRI | PstI | Low copy number plasmid, BB vector | ||||
| D73 | pSB2K3 ES open vector | MP16 pSB2K3::attP | EcoRI | SpeI | Comparison of digestion efficiency between Pst and Spe in NEB2 | ||||
| D75 | AraC-pBAD-RBS-CRE FI | L26.2 AraC-pBAD-RBS-CRE | EcoRI | SpeI | FI, Comparison of digestion efficiency between Pst and Spe in NEB2 (see D56) | ||||
