Melbourne/Secondary Reagent LB
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[[Melbourne/Protocols for Secondary Reagents|<return to list of secondary methods>]] [[Melbourne| <team home page>]] | [[Melbourne/Protocols for Secondary Reagents|<return to list of secondary methods>]] [[Melbourne| <team home page>]] | ||
===Preparation of LB=== | ===Preparation of LB=== | ||
| + | |||
===Uses=== | ===Uses=== | ||
| - | === | + | *Culturing Bacteria |
| - | * | + | *Making [[Melbourne/Secondary Reagent Agar Plates|LB-Agar Plates]] |
| + | |||
| + | ===Ingredients:=== | ||
| + | For 1L: | ||
| + | |||
| + | *10g [[Melbourne/primary Bacto-tryptone|Bacto-tryptone]] | ||
| + | *5g [[Melbourne/primary Yeast Extract|Yeast Extract]] | ||
| + | *10g [[Melbourne/primary Nacl|NaCl]] | ||
| + | *[[Melbourne/primary NaOH|NaOH]] to adjust pH | ||
| + | |||
===Method:=== | ===Method:=== | ||
| - | # | + | #Place Ingredients in a plastic container and add sterile water to the desired volume. |
| + | #Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5. | ||
Latest revision as of 11:02, 29 September 2007
<return to list of secondary methods> <team home page>
Contents |
Preparation of LB
Uses
- Culturing Bacteria
- Making LB-Agar Plates
Ingredients:
For 1L:
- 10g Bacto-tryptone
- 5g Yeast Extract
- 10g NaCl
- NaOH to adjust pH
Method:
- Place Ingredients in a plastic container and add sterile water to the desired volume.
- Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5.
