NYMU Taipei/Lab Notes/2007 10 2
From 2007.igem.org
(Difference between revisions)
(→TATA_INSA, TATB_INSB, TATD_IDE and OmpRBS GEL check after PCR) |
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Line 11: | Line 11: | ||
** lane 17: 100bp ladder | ** lane 17: 100bp ladder | ||
** lane 18: OmpRBS (PCR 6) | ** lane 18: OmpRBS (PCR 6) | ||
+ | == re-culture pOmpC and pCinRHSL == | ||
+ | == digest TATA_INSA, TATB_INSB, OmpRBS, CinR+HSL+D-term |
Revision as of 14:19, 2 October 2007
TATA_INSA, TATB_INSB, TATD_IDE and OmpRBS GEL check after PCR
- from left to right
- lane 1: 100bp ladder
- lane 2-4: TATA_INSA (PCR 1+2)
- lane 5-7: TATB_INSB (PCR 3+4)
- lane 8-12: OmpRBS (PCR 6)
- lane 13: 1kb ladder
- lane 14-16: TATD_IDE (PCR 5+7)
- lane 17: 100bp ladder
- lane 18: OmpRBS (PCR 6)
re-culture pOmpC and pCinRHSL
== digest TATA_INSA, TATB_INSB, OmpRBS, CinR+HSL+D-term