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Revision as of 06:22, 3 October 2007 (view source) Blent (Talk | contribs) (→digest TATA_INSA, TATB_INSB, OmpRBS, CinR+HSL+D-term) ← Older edit		Revision as of 06:23, 3 October 2007 (view source) Blent (Talk | contribs) (→System 1) Newer edit →
Line 20:		Line 20:
	** TATB_INSB		** TATB_INSB
	** CinR+HSL+D-term		** CinR+HSL+D-term
		+	* (pOmpC + TATA_INSA) + (TATB_INSB + CinR+HSL+D-term)
		+	** (pOmpC + TATA_INSA)
		+	** (TATB_INSB + CinR+HSL+D-term)
		+
	=== System 2 ===		=== System 2 ===
	* pCinRHSL + OmpRBS		* pCinRHSL + OmpRBS

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Revision as of 06:23, 3 October 2007

Contents 1 TATA_INSA, TATB_INSB, TATD_IDE and OmpRBS GEL check after PCR 2 re-culture pOmpC and pCinRHSL 3 digestion for assembly 3.1 System 1 3.2 System 2

TATA_INSA, TATB_INSB, TATD_IDE and OmpRBS GEL check after PCR

• from left to right
  • lane 1: 100bp ladder
  • lane 2-4: TATA_INSA (PCR 1+2)
  • lane 5-7: TATB_INSB (PCR 3+4)
  • lane 8-12: OmpRBS (PCR 6)
  • lane 13: 1kb ladder
  • lane 14-16: TATD_IDE (PCR 5+7)
  • lane 17: 100bp ladder
  • lane 18: OmpRBS (PCR 6)

re-culture pOmpC and pCinRHSL

digestion for assembly

System 1

• pOmpC + TATA_INSA
  • pOmpC
  • TATA_INSA
• TATB_INSB + CinR+HSL+D-term
  • TATB_INSB
  • CinR+HSL+D-term
• (pOmpC + TATA_INSA) + (TATB_INSB + CinR+HSL+D-term)
  • (pOmpC + TATA_INSA)
  • (TATB_INSB + CinR+HSL+D-term)

System 2

• pCinRHSL + OmpRBS
  • pCinRHSL
  • OmpRBS
• (pCinRHSL + OmpRBS) + TATD_IDE
  • (pCinRHSL + OmpRBS)
  • TATD_IDE
• [(pCinRHSL + OmpRBS) + TATD_IDE] + D-term
  • [(pCinRHSL + OmpRBS) + TATD_IDE]
  • D-term