NYMU Taipei/Lab Notes/2007 10 2
From 2007.igem.org
(Difference between revisions)
(→System 1) |
(→re-culture pOmpC and pCinRHSL) |
||
| Line 12: | Line 12: | ||
** lane 18: OmpRBS (PCR 6) | ** lane 18: OmpRBS (PCR 6) | ||
== re-culture pOmpC and pCinRHSL == | == re-culture pOmpC and pCinRHSL == | ||
| + | * Because of the low concentration of pOmpC and pCinRHSL, the liquid culture is performed again to gain larger concentration | ||
| + | * concentrations | ||
| + | ** pCinRHSL (0.05 ug/uL by pett, 0.05 and 0.08 by 海威) | ||
| + | ** pOmpC #1 (run out off in previous experiment) | ||
| + | ** pOmpC #2 (0.02 ug/uL by pett, 0.04 and 0.05 by 海威) | ||
| + | ** pOmpC #3 (0.04 and 0.23 by 海威) | ||
| + | ** pOmpC #4 (0.04 and 0.08 by 海威) | ||
| + | |||
== digestion for assembly == | == digestion for assembly == | ||
=== System 1 === | === System 1 === | ||
Revision as of 06:30, 3 October 2007
Contents |
TATA_INSA, TATB_INSB, TATD_IDE and OmpRBS GEL check after PCR
- from left to right
- lane 1: 100bp ladder
- lane 2-4: TATA_INSA (PCR 1+2)
- lane 5-7: TATB_INSB (PCR 3+4)
- lane 8-12: OmpRBS (PCR 6)
- lane 13: 1kb ladder
- lane 14-16: TATD_IDE (PCR 5+7)
- lane 17: 100bp ladder
- lane 18: OmpRBS (PCR 6)
re-culture pOmpC and pCinRHSL
- Because of the low concentration of pOmpC and pCinRHSL, the liquid culture is performed again to gain larger concentration
- concentrations
- pCinRHSL (0.05 ug/uL by pett, 0.05 and 0.08 by 海威)
- pOmpC #1 (run out off in previous experiment)
- pOmpC #2 (0.02 ug/uL by pett, 0.04 and 0.05 by 海威)
- pOmpC #3 (0.04 and 0.23 by 海威)
- pOmpC #4 (0.04 and 0.08 by 海威)
digestion for assembly
System 1
- pOmpC + TATA_INSA
- pOmpC
- TATA_INSA
- TATB_INSB + CinR+HSL+D-term
- TATB_INSB
- CinR+HSL+D-term
- (pOmpC + TATA_INSA) + (TATB_INSB + CinR+HSL+D-term)
- (pOmpC + TATA_INSA)
- (TATB_INSB + CinR+HSL+D-term)
System 2
- pCinRHSL + OmpRBS
- pCinRHSL
- OmpRBS
- (pCinRHSL + OmpRBS) + TATD_IDE
- (pCinRHSL + OmpRBS)
- TATD_IDE
- [(pCinRHSL + OmpRBS) + TATD_IDE] + D-term
- [(pCinRHSL + OmpRBS) + TATD_IDE]
- D-term
