NYMU Taipei/Lab Notes/2007 10 3

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(Difference between revisions)
(→GEL check for digestions)
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==GEL check for digestions==
==GEL check for digestions==
-
* TATA_INSA
+
=== System 1 ===
-
* TATB_INSB
+
* pOmpC + TATA_INSA
-
* TATD_IDE
+
** pOmpC ('''re-cultured, 10/2''')
-
* OmpRBS
+
** TATA_INSA ('''X.P. digestion, 10/2''')
 +
* TATB_INSB + CinR+HSL+D-term
 +
** TATB_INSB ('''E.S. digestion, 10/2''')
 +
** CinR+HSL+D-term ('''E. digestion, 10/2''')
 +
* (pOmpC + TATA_INSA) + (TATB_INSB + CinR+HSL+D-term)
 +
** (pOmpC + TATA_INSA)
 +
** (TATB_INSB + CinR+HSL+D-term)
 +
 
 +
=== System 2 ===
 +
* pCinRHSL + OmpRBS
 +
** pCinRHSL ('''re-cultured, 10/2''')
 +
** OmpRBS ('''X. P. digestion, 10/2''')
 +
* (pCinRHSL + OmpRBS) + TATD_IDE
 +
** (pCinRHSL + OmpRBS)
 +
** TATD_IDE
 +
* [(pCinRHSL + OmpRBS) + TATD_IDE] + D-term
 +
** [(pCinRHSL + OmpRBS) + TATD_IDE]
 +
** D-term

Revision as of 13:03, 3 October 2007

GEL check for digestions

System 1

  • pOmpC + TATA_INSA
    • pOmpC (re-cultured, 10/2)
    • TATA_INSA (X.P. digestion, 10/2)
  • TATB_INSB + CinR+HSL+D-term
    • TATB_INSB (E.S. digestion, 10/2)
    • CinR+HSL+D-term (E. digestion, 10/2)
  • (pOmpC + TATA_INSA) + (TATB_INSB + CinR+HSL+D-term)
    • (pOmpC + TATA_INSA)
    • (TATB_INSB + CinR+HSL+D-term)

System 2

  • pCinRHSL + OmpRBS
    • pCinRHSL (re-cultured, 10/2)
    • OmpRBS (X. P. digestion, 10/2)
  • (pCinRHSL + OmpRBS) + TATD_IDE
    • (pCinRHSL + OmpRBS)
    • TATD_IDE
  • [(pCinRHSL + OmpRBS) + TATD_IDE] + D-term
    • [(pCinRHSL + OmpRBS) + TATD_IDE]
    • D-term
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