NYMU Taipei/Lab Notes/2007 10 3

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(→three digestion for assembly)
 
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==GEL check for digestions==
==GEL check for digestions==
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* TATA_INSA
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=== System 1 ===
-
* TATB_INSB
+
* TATA_INSA ('''X.P. digestion, 10/2''')
-
* TATD_IDE
+
* TATB_INSB ('''E.S. digestion, 10/2''')
-
* OmpRBS
+
* CinR+HSL+D-term ('''E. digestion, 10/2''')
 +
=== System 2 ===
 +
* OmpRBS ('''X. P. digestion, 10/2''')
 +
[[Image:NYMU Taipei 20071003 TATA INSA,TATB INSB,OmpRBS,TATD IDE digestion check.jpg]]
 +
* lane1  : 1kd ladder
 +
* lane2-3: TATA_INSA (D. XP)
 +
* lane3-4: TATB_INSB (D. ES)
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* lane5-6: OmpRBS (D. XP)
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* lane7  : TATD_IDE (D. E)
 +
== three digestion for assembly ==
 +
* check [https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_2#digestion_for_assembly here]
 +
== next tasks ==
 +
* pOmpC + TATA_INSA
 +
* TATB_INSB + CinR+HSL+D-term
 +
* pCinRHSL + OmpRBS

Latest revision as of 13:55, 3 October 2007

Contents

GEL check for digestions

System 1

  • TATA_INSA (X.P. digestion, 10/2)
  • TATB_INSB (E.S. digestion, 10/2)
  • CinR+HSL+D-term (E. digestion, 10/2)

System 2

  • OmpRBS (X. P. digestion, 10/2)

NYMU Taipei 20071003 TATA INSA,TATB INSB,OmpRBS,TATD IDE digestion check.jpg

  • lane1  : 1kd ladder
  • lane2-3: TATA_INSA (D. XP)
  • lane3-4: TATB_INSB (D. ES)
  • lane5-6: OmpRBS (D. XP)
  • lane7  : TATD_IDE (D. E)

three digestion for assembly

next tasks

  • pOmpC + TATA_INSA
  • TATB_INSB + CinR+HSL+D-term
  • pCinRHSL + OmpRBS
Retrieved from "http://2007.igem.org/wiki/index.php/NYMU_Taipei/Lab_Notes/2007_10_3"