Toronto/Lab Notebook
From 2007.igem.org
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<font size=4>< [[August]] | [[September]] | [[Toronto/Lab Notebook|Current]] ></font> | <font size=4>< [[August]] | [[September]] | [[Toronto/Lab Notebook|Current]] ></font> | ||
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| + | == Oct. 2, 2007 == | ||
| + | |||
| + | Natalie | ||
| + | |||
| + | SUMMARY: | ||
| + | |||
| + | - Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB | ||
| + | - NOTE: N1 = J34100 C + S01640 B + B0015 | ||
| + | |||
| + | TO DO: | ||
| + | |||
| + | - Miniprep all above parts and length check | ||
| + | - Transform: | ||
| + | J06801 (2005) - it's in the source box, | ||
| + | B0034 - also in | ||
== Oct. 1, 2007 == | == Oct. 1, 2007 == | ||
Revision as of 15:25, 3 October 2007
Oct. 2, 2007
Natalie
SUMMARY:
- Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB - NOTE: N1 = J34100 C + S01640 B + B0015
TO DO:
- Miniprep all above parts and length check - Transform:
J06801 (2005) - it's in the source box,
B0034 - also in
Oct. 1, 2007
Time: 3:45 PM - 8:30 PM
Yusuf, Irina
Summary:
- made 5 KAN plates
- made 2 AMP+KAN plates
- made 300 mL LB
Tranformation of following parts:
- I13033 -> KAN
- E0433 -> KAN
- S03140 -> AMP
- J04500 -> KAN
- J31003 -> AMP
- (J23100C + S01640B)+ B0015 -> KAN
Used 4uL of DNA for all transformations
- tried to do DNA extraction for J06501 but somebody already extracted it (wasn't in the well)
- couldn't find it in the igem dna box as well !!!
TO DO:
- MP o/n for the above parts
