Valencia/Adversities

From 2007.igem.org

Revision as of 15:20, 7 October 2007

Here it is, here it is not (part II)

if you think that an improbable fact is way improbable to happen twice... read this...

we couldn't believe our eyes when the fluorescence from the ethidium bromide just disappeared in front of us...

those weird things that happen from time to time, that are in open confrontation to the commen sense, are an issue for the functional, positivist kind of science that is made nowadays in the lab -arnau

Waiting for Mr. Cell

the commercial cells were meant to arrive on the first of august, but they arrived on day 8. we tried to tranform home made DH5a and XL1-blue with no colonies at the end of the day...

but everything looks better since we use the commercial ones...

A strange visitor

august 15 was a national holiday in Spain, so everything was closed. even kindergarden. so we had a visit from a three-year-old child that could not be still for five minutes.

we had to take all our patience out to do our minipreps and purifications with a half-meter tall child asking 'why' every while...

No bacteria, again

waiting for the commercial cells, we tried to work with home-made chemically competent DH5-α... the efficiency was lower than zero, if that could be possible...

Kanamicine resistant, only?

in order to do the backbone shift, we had to transform some kanamicine resistant (ONLY) plasmid. we did not worry about the Biobrick, as we were to excise it to use it with our constructions...

well, we have tried three Biobricks up to now, and we have witnessed two things: they grew in ampicillin AND kanamicine (and they were marked as only kanamicine on the registry) or they did not grow AT ALL...