Melbourne/Lab Notebook Weeks 5-8
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*[[Melbourne/Colony PCRl|Colony PCRed]] using [[Melbourne/primary vf2|vf2]] and [[Melbourne/primary vR|vR]] primers the transformations from 20/7. 3 colonies from ligation plate and one from plasmid control plate. | *[[Melbourne/Colony PCRl|Colony PCRed]] using [[Melbourne/primary vf2|vf2]] and [[Melbourne/primary vR|vR]] primers the transformations from 20/7. 3 colonies from ligation plate and one from plasmid control plate. | ||
Revision as of 12:29, 8 October 2007
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Contents |
Week 5
23 July 2007
- Colony PCRed using vf2 and vR primers the transformations from 20/7. 3 colonies from ligation plate and one from plasmid control plate.
24 July 2007
- Ran Gel from PCRs (23/7) at 100V.
-results: colony 3 didn't give product.
- Streaked out some cells from index plate (No.3).
25 July 2007
26 July 2007
27 July 2007
Week 6
30 July 2007
31 July 2007
1 Aug 2007
2 Aug 2007
3 Aug 2007
4 Aug 2007
Week 7
5 Aug 2007
6 Aug 2007
7 Aug 2007
8 Aug 2007
9 Aug 2007
10 Aug 2007
11 Aug 2007
Week 8
12 Aug 2007
13 Aug 2007
14 Aug 2007
15 Aug 2007
16 Aug 2007
17 Aug 2007
18 Aug 2007
