Melbourne/Lab BL Notebook/PCR1

From 2007.igem.org

(Difference between revisions)
Line 10: Line 10:
*1ul Template ([[Melbourne/pJS010|pJS010]])
*1ul Template ([[Melbourne/pJS010|pJS010]])
*0.4ul Pfx Platinum (Invitrogen)
*0.4ul Pfx Platinum (Invitrogen)
-
*32.5ul ddH<sub>_{2}</sub>
+
*32.5ul ddH<sub>2</sub>O
 +
 
 +
----
 +
50ul Total

Revision as of 14:44, 10 October 2007

Protocol for PCR reactions A-G (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

  • 5ul 10x buffer
  • 5ul Enhancer
  • 0.6ul dNTPs (25mM stock)
  • 2.5ul MgSO4 (Supplied in PCR kit)
  • 1.5ul Primer I (10uM)
  • 1.5ul Primer II (10uM)
  • 1ul Template (pJS010)
  • 0.4ul Pfx Platinum (Invitrogen)
  • 32.5ul ddH2O

50ul Total

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