Melbourne/Lab BL Notebook/PCR1

From 2007.igem.org

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Protocol for PCR reactions A-G (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.
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=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.
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=PCR mix=
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====PCR mix====
*5ul 10x buffer
*5ul 10x buffer
*5ul Enhancer
*5ul Enhancer

Revision as of 14:44, 10 October 2007

=Protocol for PCR reactions A~G= (and -ve control) amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix

  • 5ul 10x buffer
  • 5ul Enhancer
  • 0.6ul dNTPs (25mM stock)
  • 2.5ul MgSO4 (Supplied in PCR kit)
  • 1.5ul Primer I (10uM)
  • 1.5ul Primer II (10uM)
  • 1ul Template (pJS010)
  • 0.4ul Pfx Platinum (Invitrogen)
  • 32.5ul ddH2O

50ul Total

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