Melbourne/Lab BL Notebook/PCR1
From 2007.igem.org
(Difference between revisions)
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| √2 | | √2 | ||
|- | |- | ||
| - | | | + | | 50ul Total |
| <math>\sqrt{1-e^2}</math> | | <math>\sqrt{1-e^2}</math> | ||
| <code><nowiki>&radic;(1&minus;''e''&sup2;)</nowiki></code> | | <code><nowiki>&radic;(1&minus;''e''&sup2;)</nowiki></code> | ||
Revision as of 14:55, 10 October 2007
Protocol for PCR reactions A~G
For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.
| PCR mix | PCR program | ||
|---|---|---|---|
| 5ul 10x buffer\\
5ul Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer I (10uM) 1.5ul Primer II (10uM) 1ul Template (pJS010) 0.4ul Pfx Platinum (Invitrogen) 32.5ul ddH2O | |||
<math>\sqrt{2}</math>
| <math>\sqrt{2}</math> | √2
| √2 |
| 50ul Total | <math>\sqrt{1-e^2}</math> | √(1−''e''²)
| √(1−e²) |
|-
PCR mix
|5ul 10x buffer\\
5ul Enhancer
0.6ul dNTPs (25mM stock)
2.5ul MgSO4 (Supplied in PCR kit)
1.5ul Primer I (10uM)
1.5ul Primer II (10uM)
1ul Template (pJS010)
0.4ul Pfx Platinum (Invitrogen)
32.5ul ddH2O
---
50ul Total | |-
