From 2007.igem.org

Revision as of 14:55, 10 October 2007 (view source) Alisa (Talk | contribs) ← Older edit		Revision as of 14:56, 10 October 2007 (view source) Alisa (Talk | contribs) Newer edit →
Line 25:		Line 25:
	32.5ul ddH<sub>2</sub>O		32.5ul ddH<sub>2</sub>O
	|-		|-
-	| <code><nowiki><math>\sqrt{2}</math></nowiki></code>	+
-	| <math>\sqrt{2}</math>	+
-	| <code><nowiki>&amp;radic;2</nowiki></code>	+
-	| &radic;2	+
-	|-	+
	| 50ul Total		| 50ul Total
-	| <math>\sqrt{1-e^2}</math>
-	| <code><nowiki>&amp;radic;(1&amp;minus;''e''&amp;sup2;)</nowiki></code>
-	| &radic;(1&minus;''e''&sup2;)
	|}		|}

---

Revision as of 14:56, 10 October 2007

Protocol for PCR reactions A~G

For amplifying the photoreceptor and transmembrane domains of NpSopII-NpHtrII.

PCR mix	PCR program
5ul 10x buffer\\ 5ul Enhancer 0.6ul dNTPs (25mM stock) 2.5ul MgSO4 (Supplied in PCR kit) 1.5ul Primer I (10uM) 1.5ul Primer II (10uM) 1ul Template (pJS010) 0.4ul Pfx Platinum (Invitrogen) 32.5ul ddH2O
50ul Total

|-

PCR mix

|5ul 10x buffer\\

5ul Enhancer

0.6ul dNTPs (25mM stock)

2.5ul MgSO4 (Supplied in PCR kit)

1.5ul Primer I (10uM)

1.5ul Primer II (10uM)

1ul Template (pJS010)

0.4ul Pfx Platinum (Invitrogen)

32.5ul ddH₂O

---

50ul Total | |-